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Prepublished online as a Blood First Edition Paper on August 28, 2003; DOI 10.1182/blood-2003-07-2465. This Article Full Text (PDF) All Versions of this Article: 2003-07-2465v1 102/13/4487    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Duramad, O. Articles by Barrat, F. J Search for Related Content PubMed PubMed Citation Articles by Duramad, O. Articles by Barrat, F. J Social Bookmarking                   What's this? Submitted July 22, 2003 Accepted August 24, 2003 IL-10 regulates plasmacytoid dendritic cell response to CpG containing immunostimulatory sequences Omar Duramad, Karen L Fearon, Jean H Chan, Holger Kanzler, Jason D Marshall, Robert L Coffman, and Franck J Barrat* Dynavax Technologies Corp., Berkeley, CA, USA * Corresponding author; email: fbarrat{at}dvax.com. Immunostimulatory sequences (ISS) are short oligonucleotides containing unmethylated CpG dinucleotides that stimulate innate immune responses through Toll-like receptor-9 on B cells and plasmacytoid dendritic cell precursors (PDC). The anti-inflammatory cytokine IL-10 is predicted to be a potent inhibitor of many of the activities described for ISS, and this may impact the use of ISS in disease states characterized by elevated IL-10. As the activities of ISS on PDC are central to many clinical applications of ISS, we have studied the effects of IL-10 on PDC stimulation by three distinct classes of ISS. IL-10 inhibited cytokine production and survival of ISS-activated PDC, however IL-12 induction was much more sensitive to inhibition than IFN-a induction. Within the PDC population are cells that respond to ISS by producing either IL-12 or IFN-, but not both cytokines. IL-12 producing PDC require costimulation through CD40 and appear more mature than IFN-a-producing PDC. The three distinct classes of ISS differed with respect to induction of PDC maturation and T cell priming capacity. IL-10 regulated PDC activation, but did not inhibit the subsequent T cell priming ability of PDC already activated by ISS. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C. Sadaka, M.-A. Marloie-Provost, V. Soumelis, and P. Benaroch Developmental regulation of MHC II expression and transport in human plasmacytoid-derived dendritic cells Blood, March 5, 2009; 113(10): 2127 - 2135. [Abstract] [Full Text] [PDF] B. G. 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