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Blood, 1 June 2004, Vol. 103, No. 11, pp. 4344-4352. Prepublished online as a Blood First Edition Paper on February 19, 2004; DOI 10.1182/blood-2003-07-2534. This Article Full Text (PDF) All Versions of this Article: 2003-07-2534v1 103/11/4344    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Chen, B. J Articles by Chao, N. J Search for Related Content PubMed PubMed Citation Articles by Chen, B. J Articles by Chao, N. J Social Bookmarking                   What's this? Submitted July 25, 2003 Accepted February 6, 2004 Hematopoietic Stem Cell Dose Correlates with the Speed of Immune Reconstitution after Stem Cell Transplantation Benny J Chen*, Xiuyu Cui, Gregory D Sempowski, Jos Domen, and Nelson J Chao Bone Marrow Transplantation Program, Duke University Medical Center, Durham, NC, USA; Department of Medicine, Duke University Medical Center, Durham, NC, USA Department of Medicine, Duke University Medical Center, Durham, NC, USA; Human Vaccine Institute, Duke University Medical Center, Durham, NC, USA Bone Marrow Transplantation Program, Duke University Medical Center, Durham, NC, USA; Department of Medicine, Duke University Medical Center, Durham, NC, USA; Department of Immunology, Duke University Medical Centeer, Durham, NC, USA * Corresponding author; email: chen0032{at}mc.duke.edu. In the current study, we tested whether higher numbers of hematopoietic stem cells correlate with the speed of immune reconstitution in a congenic transplantation model (C57BL/6, CD45.1, Thy1.1 C57BL/6, CD45.2, Thy1.2+) using purified hematopoietic stem cells (c-Kit+Thy1.1lowLin-/lowSca-1+). Three different doses of stem cells (400, 1000, 5000) were used. Phenotypic analyses in peripheral blood and spleen demonstrated that more stem cells infused are associated with more rapid regeneration of T cells (CD4+, CD8+, naive CD4+, naive CD8+) and B cells up to 10 weeks after transplantation. The numbers of T and B cells became equivalent among different dose groups at week +14. Production of interleukin-2 and interferon per T cell was similar regardless of stem cell dose even when tested at the time when there were significant differences in peripheral T cell counts. The improved immune recovery was attributed to a more rapid regeneration of donor-type immune cells. Higher numbers of total thymocytes and signal joint T cell receptor excision circles were observed in the higher dose stem cell recipients, suggesting accelerated regeneration of T cells was due to enhanced thymopoiesis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: K. M. Williams, P. J. Lucas, C. V. Bare, J. Wang, Y.-W. Chu, E. Tayler, V. Kapoor, and R. E. Gress CCL25 increases thymopoiesis after androgen withdrawal Blood, October 15, 2008; 112(8): 3255 - 3263. [Abstract] [Full Text] [PDF] Y.-W. Chu, S. Schmitz, B. Choudhury, W. Telford, V. Kapoor, S. Garfield, D. Howe, and R. E. Gress Exogenous insulin-like growth factor 1 enhances thymopoiesis predominantly through thymic epithelial cell expansion Blood, October 1, 2008; 112(7): 2836 - 2846. [Abstract] [Full Text] [PDF] J. Mariotti, J. Foley, U. Jung, T. Borenstein, N. Kantardzic, S. Han, J. T. Hanson, E. Wong, N. Buxhoeveden, J. B. Trepel, et al. Ex Vivo Rapamycin Generates Apoptosis-Resistant Donor Th2 Cells That Persist In Vivo and Prevent Hemopoietic Stem Cell Graft Rejection J. Immunol., January 1, 2008; 180(1): 89 - 105. [Abstract] [Full Text] [PDF] M. H. Dallas, B. Varnum-Finney, P. J. Martin, and I. D. Bernstein Enhanced T-cell reconstitution by hematopoietic progenitors expanded ex vivo using the Notch ligand Delta1 Blood, April 15, 2007; 109(8): 3579 - 3587. [Abstract] [Full Text] [PDF] B. J. Chen, D. Deoliveira, X. Cui, N. T. Le, J. Son, J. F. Whitesides, and N. J. Chao Inability of memory T cells to induce graft-versus-host disease is a result of an abortive alloresponse Blood, April 1, 2007; 109(7): 3115 - 3123. [Abstract] [Full Text] [PDF] N. J. Chao, S. G. Emerson, and K. I. Weinberg Stem Cell Transplantation (Cord Blood Transplants) Hematology, January 1, 2004; 2004(1): 354 - 371. [Abstract] [Full Text] [PDF]

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