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Blood, 1 May 2004, Vol. 103, No. 9, pp. 3590-3598. Prepublished online as a Blood First Edition Paper on January 8, 2004; DOI 10.1182/blood-2003-08-2827. This Article Full Text (PDF) All Versions of this Article: 2003-08-2827v1 103/9/3590    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Panoskaltsis-Mortari, A. Articles by Blazar, B. R Search for Related Content PubMed PubMed Citation Articles by Panoskaltsis-Mortari, A. Articles by Blazar, B. R Social Bookmarking                   What's this? Submitted August 18, 2003 Accepted January 2, 2004 In Vivo Imaging of Graft-Versus-Host-Disease (GHVD) In Mice Angela Panoskaltsis-Mortari*, Andrew Price, John R Hermanson, Elizabeth Taras, Chris Lees, Jonathan S Serody, and Bruce R Blazar Pediatric Hematology, Oncology, Blood and Marrow Transplant and Cancer Center, University of Minnesota, Minneapolis, MN, USA Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC, USA * Corresponding author; email: panos001{at}umn.edu. We have developed a mouse system in which to track the migration and homing of cells in a setting of BMT-induced graft-versus-host disease (GVHD) following systemic infusion using eGFP transgenic cells and a simple application of a fluorescence stereomicroscope outfitted with a color CCD camera. Whole body images of anesthetized mice taken at various time points after cell infusion revealed early migration of allogeneic cells to peripheral lymphoid organs with later infiltration of GVHD target organs. Localization of GFP Tg cells could be seen through the skin of shaved mice and internal organs were easily discernable. Following allogeneic or syngeneic GFP Tg cell infusion, representative mice were dissected to better-visualize deeper internal organs and tissues. Infusion of different cell populations revealed distinct homing patterns and this method also provided a simple way to identify the critical time points for expansion of the transplanted cells in various organs. This simple application of the fluorescence stereomicroscope will be valuable for GVHD and graft-versus-tumor studies in which visualization of cellular migration, expansion and cell-cell interactions will be more informative when analyzed by such an intravital method. 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