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Blood, 1 March 2004, Vol. 103, No. 5, pp. 1747-1754. Prepublished online as a Blood First Edition Paper on October 30, 2003; DOI 10.1182/blood-2003-08-2828. This Article Full Text (PDF) All Versions of this Article: 2003-08-2828v1 103/5/1747    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Wan, T. Articles by Cao, X. Search for Related Content PubMed PubMed Citation Articles by Wan, T. Articles by Cao, X. Social Bookmarking                   What's this? Submitted August 18, 2003 Accepted October 22, 2003 Novel heat shock protein Hsp70L1 activates dendritic cells and acts as a Th1 polarizing adjuvant Tao Wan, Xiangyang Zhou, Guoyou Chen, Huazhang An, Taoyong Chen, Weiping Zhang, Shuxun Liu, Yingming Jiang, Feng Yang, Yanfeng Wu, and Xuetao Cao* Institute of Immunology, Second Military Medical Universty, Shanghai, China * Corresponding author; email: caoxt{at}public3.sta.net.cn. Heat shock proteins (HSPs) are reported to act as effective adjuvants to elicit anti-tumor and anti-infection immunity. Here, we report that Hsp70-like protein 1 (Hsp70L1), a novel HSP derived from human dendritic cells (DC) has potent adjuvant effects that polarize responses towards Th1. With a calculated molecular weight of 54.8 kD, Hsp70L1 is smaller in size than Hsp70, but resembles it both structurally and functionally. Hsp70L1 shares common receptors on DC with Hsp70 and can interact with DC, promoting DC maturation, and stimulating secretion of the proinflammatory cytokines IL-12p70, IL-1 and TNF- and the chemokines IP-10 , MIP-1, MIP-1 and RANTES. The induction of IP-10 secretion by Hsp70L1 is not shared by Hsp70, and other functional differences include more potent stimulation of DC IL-12p70, CC-chemokine, and CCR7 and CXCR4 expression by Hsp70L1. Immunization of mice with the hybrid peptide Hsp70L1-OVA257-264 induces an OVA257-264-specific Th1 response and CTL that results in significant inhibition of E.G7-OVA tumor growth. The ability of Hsp70L1 to activate DC indicates its potential as a novel adjuvant for use with peptide immunizations; the Hsp70L1 antigen peptide hybrid may serve as a more effective vaccine for the control of cancer and infectious diseases. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: T. 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