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Blood, 15 May 2004, Vol. 103, No. 10, pp. 3854-3859. Prepublished online as a Blood First Edition Paper on January 22, 2004; DOI 10.1182/blood-2003-08-2923. This Article Full Text (PDF) All Versions of this Article: 2003-08-2923v1 103/10/3854    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Laskus, T. Articles by Rakela, J. Search for Related Content PubMed PubMed Citation Articles by Laskus, T. Articles by Rakela, J. Social Bookmarking                   What's this? Submitted September 2, 2003 Accepted January 13, 2004 Human immunodeficiency virus facilitates infection/replication of hepatitis C virus in native human macrophages Tomasz Laskus, Marek Radkowski, Joanna Jablonska, Karen Kibler, Jeffrey Wilkinson, Debra Adair, and Jorge Rakela* Medicine, Mayo Clinic Scottsdale, Scottsdale, AZ, USA Medicine, Mayo Clinic Scottsdale, Scottsdale, AZ, USA; Institute of Infectious Diseases, Medical Academy Warsaw, Warsaw, Poland Institute of Infectious Diseases, Medical Academy Warsaw, Warsaw, Poland * Corresponding author; email: rakela.jorge{at}mayo.edu. Hepatitis C virus (HCV) was found to replicate in monocytes/macrophages particularly in patients with human immunodeficiency virus type 1 (HIV) infection. This study was undertaken to determine whether HIV facilitates HCV infection of native human macrophages in vitro. Monocytes/macrophages were collected from healthy donors, infected with HIV M-tropic molecular clone, and then exposed to HCV-positive sera. Presence of positive and negative HCV RNA strands was determined with a novel strand-specific quantitative real-time RT-PCR. Preceding as well as near simultaneous infection with HIV made the macrophages more susceptible to infection with HCV; in particular, HCV RNA negative strand was detectable almost exclusively in the setting of concomitant HIV infection. Furthermore, HCV RNA load correlated with HIV replication level in the early stage of infection. The ratio of positive to negative strand in macrophages was lower than in control liver samples. HIV infection was also found to facilitate HCV replication in Daudi B-cell line with engineered CD4 expression. It seems that HIV infection can facilitate replication of HCV in monocytes/macrophages either by rendering cells more susceptible to HCV infection or by increasing HCV replication. This could explain the presence of extrahepatic HCV replication in HIV-coinfected individuals. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. Wilkinson, M. Radkowski, and T. Laskus Hepatitis C Virus Neuroinvasion: Identification of Infected Cells J. Virol., February 1, 2009; 83(3): 1312 - 1319. [Abstract] [Full Text] [PDF] S. A. MacParland, T. N. Q. Pham, S. A. Gujar, and T. I. Michalak De novo infection and propagation of wild-type Hepatitis C virus in human T lymphocytes in vitro J. Gen. Virol., December 1, 2006; 87(12): 3577 - 3586. [Abstract] [Full Text] [PDF] P. Bare, I. Massud, C. Parodi, L. Belmonte, G. Garcia, M. C. Nebel, M. Corti, M. T. Pinto, R. P. Bianco, M. M. Bracco, et al. Continuous release of hepatitis C virus (HCV) by peripheral blood mononuclear cells and B-lymphoblastoid cell-line cultures derived from HCV-infected patients J. Gen. Virol., June 1, 2005; 86(6): 1717 - 1727. [Abstract] [Full Text] [PDF] W. G. van Gorp and C. H. Hinkin Triple trouble: Cognitive deficits from hepatitis C, HIV, and methamphetamine Neurology, April 26, 2005; 64(8): 1328 - 1329. [Full Text] [PDF]

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