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Blood, 15 May 2004, Vol. 103, No. 10, pp. 3798-3804. Prepublished online as a Blood First Edition Paper on December 4, 2003; DOI 10.1182/blood-2003-08-2952. This Article Full Text (PDF) All Versions of this Article: 2003-08-2952v1 103/10/3798    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Szczepanski, T. Articles by van Dongen, J. J M Search for Related Content PubMed PubMed Citation Articles by Szczepanski, T. Articles by van Dongen, J. J M Social Bookmarking                   What's this? Submitted August 29, 2003 Accepted November 30, 2003 V2-J gene rearrangements are frequent in precursor-B-acute lymphoblastic leukemia but rare in normal lymphoid cells Tomasz Szczepanski, Vincent H J van der Velden, Patricia G Hoogeveen, Maaike de Bie, Danielle C H Jacobs, Elisabeth R van Wering, and Jacques J M van Dongen* Department of Immunology, Erasmus Medical Center, University Medical Center Rotterdam, Rotterdam, The Netherlands Department of Pediatric Hematology and Oncology, Silesian Medical Academy, Zabrze, Poland Dutch Childhood Oncology Group, The Hague, The Netherlands * Corresponding author; email: j.j.m.vandongen{at}erasmusmc.nl. The frequently occurring T-cell receptor delta (TCRD) deletions in precursor-B-ALL are assumed to be mainly caused by V2-J gene rearrangements. We designed a multiplex PCR assay with 61 J primers and identified clonal V2-J gene rearrangements in 141 of 339 (41%) childhood and 8 of 22 (36%) adult precursor-B-ALL. A significant proportion (44%) of V2-J gene rearrangements in childhood precursor-B-ALL were oligoclonal. Sequence analysis showed preferential usage of the J29 gene segment in 54% of rearrangements. The remaining V2-J rearrangements used 26 other J segments, which included two additional clusters, one involving the most upstream J segments, i.e. J48 to J61 (23%), and the second cluster located around the J9 gene segment (7%). Real-time quantitative PCR studies of normal lymphoid cells showed that V2 rearrangements to upstream J segments occurred at low levels in the thymus (10-2 to 10-3), and were rare (generally below 10-3) in B-cell-precursors and mature T-cells. V2-J29 gene rearrangements were virtually absent in normal lymphoid cells. The monoclonal V2-J rearrangements in precursor-B-ALL may serve as patient-specific targets for detection of minimal residual disease, since they show high sensitivity (10-4 in most cases) and good stability (88% of rearrangements preserved at relapse). CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: N. Hagedorn, C. Acquaviva, E. Fronkova, A. von Stackelberg, A. Barth, U. zur Stadt, A. Schrauder, J. Trka, N. Gaspar, K. Seeger, et al. Submicroscopic bone marrow involvement in isolated extramedullary relapses in childhood acute lymphoblastic leukemia: a more precise definition of "isolated" and its possible clinical implications, a collaborative study of the Resistant Disease Committee of the International BFM study group Blood, December 1, 2007; 110(12): 4022 - 4029. [Abstract] [Full Text] [PDF] E. R. Panzer-Grumayer, G. Cazzaniga, V. H.J. van der Velden, L. del Giudice, M. Peham, G. Mann, C. Eckert, A. Schrauder, G. Germano, J. Harbott, et al. Immunogenotype Changes Prevail in Relapses of Young Children with TEL-AML1-Positive Acute Lymphoblastic Leukemia and Derive Mainly from Clonal Selection Clin. Cancer Res., November 1, 2005; 11(21): 7720 - 7727. [Abstract] [Full Text] [PDF]

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