SEARCH:   Advanced

Blood, 15 July 2004, Vol. 104, No. 2, pp. 462-469. Prepublished online as a Blood First Edition Paper on March 25, 2004; DOI 10.1182/blood-2003-08-2990. This Article Full Text (PDF) All Versions of this Article: 2003-08-2990v1 104/2/462    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Gilchrist, M. Articles by Befus, A D Search for Related Content PubMed PubMed Citation Articles by Gilchrist, M. Articles by Befus, A D Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted September 4, 2003 Accepted March 8, 2004 Expression, localization and regulation of nitric oxide synthase (NOS) in human mast cell lines: Effects on leukotriene production Mark Gilchrist*, Scott D McCauley, and A D Befus Institute for Systems Biology, Seattle, Washington, USA Medicine, University of Alberta, Edmonton, Alberta, Canada * Corresponding author; email: mgilchrist{at}systemsbiology.org. Nitric oxide (NO) is a potent radical produced by nitric oxide synthase (NOS), and has pleiotrophic activities in health and disease. As mast cells (MC) play a central role in both homeostasis and pathology, we investigated NOS expression and NO production in human MC populations. Endothelial NOS (eNOS) was ubiquitously expressed in both human MC lines and skin-derived MC, while neuronal NOS (nNOS) was variably expressed in the MC populations studied. The inducible (iNOS) isoform was not detected in human MC. Both growth factor-independent (HMC-1) and dependent (LAD 2) MC lines showed predominant nuclear eNOS protein localization, with weaker cytoplasmic expression. nNOS showed exclusive cytoplasmic localization in HMC-1. Activation with Ca2+ ionophore (A23187) or IgE-anti-IgE induced eNOS phosphorylation and translocation to the nucleus and nuclear and cytoplasmic NO formation. eNOS colocalizes with the leukotriene (LT) initiating enzyme 5-lipoxygenase (5-LO) in the MC nucleus. The NO donor, SNOG, inhibited, while the NOS inhibitor, L-NAME, potentiated LT release in a dose dependent manner. Thus, human MC lines produce NO in both cytoplasmic and nuclear compartments, and endogenously produced NO can regulate LT production by MC. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: T. Inoue, Y. Suzuki, T. Yoshimaru, and C. Ra Nitric oxide positively regulates Ag (I)-induced Ca2+ influx and mast cell activation: role of a nitric oxide synthase-independent pathway J. Leukoc. Biol., December 1, 2009; 86(6): 1365 - 1375. [Abstract] [Full Text] [PDF] T. Inoue, Y. Suzuki, T. Yoshimaru, and C. Ra Nitric oxide protects mast cells from activation-induced cell death: the role of the phosphatidylinositol-3 kinase-Akt-endothelial nitric oxide synthase pathway J. Leukoc. Biol., May 1, 2008; 83(5): 1218 - 1229. [Abstract] [Full Text] [PDF] M. Marcet-Palacios, M. Ulanova, F. Duta, L. Puttagunta, S. Munoz, D. Gibbings, M. Radomski, L. Cameron, I. Mayers, and A. D. Befus The Transcription Factor Wilms Tumor 1 Regulates Matrix Metalloproteinase-9 through a Nitric Oxide-Mediated Pathway J. Immunol., July 1, 2007; 179(1): 256 - 265. [Abstract] [Full Text] [PDF] R. F. Machado, A. Anthi, M. H. Steinberg, D. Bonds, V. Sachdev, G. J. Kato, A. M. Taveira-DaSilva, S. K. Ballas, W. Blackwelder, X. Xu, et al. N-terminal pro-brain natriuretic peptide levels and risk of death in sickle cell disease. JAMA, July 19, 2006; 296(3): 310 - 318. [Abstract] [Full Text] [PDF] F. Gobeil Jr., T. Zhu, S. Brault, A. Geha, A. Vazquez-Tello, A. Fortier, D. Barbaz, D. Checchin, X. Hou, M. Nader, et al. Nitric Oxide Signaling via Nuclearized Endothelial Nitric-oxide Synthase Modulates Expression of the Immediate Early Genes iNOS and mPGES-1 J. Biol. Chem., June 9, 2006; 281(23): 16058 - 16067. [Abstract] [Full Text] [PDF] R. Saini, S. Patel, R. Saluja, A. A. Sahasrabuddhe, M. P. Singh, S. Habib, V. K. Bajpai, and M. Dikshit Nitric oxide synthase localization in the rat neutrophils: immunocytochemical, molecular, and biochemical studies J. Leukoc. Biol., March 1, 2006; 79(3): 519 - 528. [Abstract] [Full Text] [PDF] A.-K. Larsson, M. Back, J. Hjoberg, and S.-E. Dahlen Inhibition of Nitric-Oxide Synthase Enhances Antigen-Induced Contractions and Increases Release of Cysteinyl-Leukotrienes in Guinea Pig Lung Parenchyma: Nitric Oxide as a Protective Factor J. Pharmacol. Exp. Ther., October 1, 2005; 315(1): 458 - 465. [Abstract] [Full Text] [PDF] M. Peters-Golden, C. Canetti, P. Mancuso, and M. J. Coffey Leukotrienes: Underappreciated Mediators of Innate Immune Responses J. Immunol., January 15, 2005; 174(2): 589 - 594. [Abstract] [Full Text] [PDF] E. J. Swindle, D. D. Metcalfe, and J. W. Coleman Rodent and Human Mast Cells Produce Functionally Significant Intracellular Reactive Oxygen Species but Not Nitric Oxide J. Biol. Chem., November 19, 2004; 279(47): 48751 - 48759. [Abstract] [Full Text] [PDF]

	Copyright © 2004 by American Society of Hematology         Online ISSN: 1528-0020