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Blood, 1 March 2004, Vol. 103, No. 5, pp. 1662-1668. Prepublished online as a Blood First Edition Paper on October 30, 2003; DOI 10.1182/blood-2003-09-3070. This Article Full Text (PDF) All Versions of this Article: 2003-09-3070v1 103/5/1662    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Peister, A. Articles by Prockop, D. J Search for Related Content PubMed PubMed Citation Articles by Peister, A. Articles by Prockop, D. J Social Bookmarking                   What's this? Submitted September 5, 2003 Accepted October 24, 2003 Adult stem cells from bone marrow (MSCs) isolated from different strains of inbred mice vary in surface epitopes, rates of proliferation, and differentiation potential Alexandra Peister, Jason A Mellad, Benjamin L Larson, Brett M Hall, Laura F Gibson, and Darwin J Prockop* Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, LA, USA Gene Therapy Center, Childrens Research Insititute, Columbus, OH, USA Department of Pediatrics and Department of Microbiology, Immunology, and Cell Biology, West Virginia University, Morgantown, WV, USA * Corresponding author; email: dprocko{at}tulane.edu. For reasons that are not apparent, it has been difficult to isolate and expand the adult stem cells referred to as mesenchymal stem cells or marrow stromal cells (MSCs) from murine bone marrow. We developed a protocol that provides rapidly expanding MSCs from five strains of inbred mice. The MSCs obtained from five different strains of mice were similar to human and rat MSCs in that they expanded more rapidly if plated at very low density, formed single-cell derived colonies, and readily differentiated into either adipocytes, chondrocytes, or mineralizing cells. However, the cells from the five strains differed in their media requirements for optimal growth, rates of propagation, and presence of the surface epitopes CD34, Sca-1 and VCAM-1. The protocol should make it possible to undertake a large number of experiments with MSCs in transgenic mice that have previously not been possible. The differences among MSCs from different strains may explain some of the conflicting data recently published on the engraftment of mouse MSCs or other bone marrow cells into non-hematopoietic tissues. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. Aslam, R. Baveja, O. D. Liang, A. 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Neumann Hematopoietic Stem Cells Reduce Postischemic Inflammation and Ameliorate Ischemic Brain Injury Stroke, October 1, 2008; 39(10): 2867 - 2875. [Abstract] [Full Text] [PDF] P. R. Crisostomo, A. M. Abarbanell, M. Wang, T. Lahm, Y. Wang, and D. R. Meldrum Embryonic stem cells attenuate myocardial dysfunction and inflammation after surgical global ischemia via paracrine actions Am J Physiol Heart Circ Physiol, October 1, 2008; 295(4): H1726 - H1735. [Abstract] [Full Text] [PDF] R. Summer and A. Fine Mesenchymal Progenitor Cell Research: Limitations and Recommendations Proceedings of the ATS, August 15, 2008; 5(6): 707 - 710. [Abstract] [Full Text] [PDF] Y.-X. Ren, F. G. Finckenstein, D. A. Abdueva, V. Shahbazian, B. Chung, K. I. Weinberg, T. J. Triche, H. Shimada, and M. J. Anderson Mouse Mesenchymal Stem Cells Expressing PAX-FKHR Form Alveolar Rhabdomyosarcomas by Cooperating with Secondary Mutations Cancer Res., August 15, 2008; 68(16): 6587 - 6597. [Abstract] [Full Text] [PDF] Y. 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Dzau Mesenchymal Stem Cells Use Integrin beta1 Not CXC Chemokine Receptor 4 for Myocardial Migration and Engraftment Mol. Biol. Cell, August 1, 2007; 18(8): 2873 - 2882. [Abstract] [Full Text] [PDF] K. Kamiya, Y. Fujinami, N. Hoya, Y. Okamoto, H. Kouike, R. Komatsuzaki, R. Kusano, S. Nakagawa, H. Satoh, M. Fujii, et al. Mesenchymal Stem Cell Transplantation Accelerates Hearing Recovery through the Repair of Injured Cochlear Fibrocytes Am. J. Pathol., July 1, 2007; 171(1): 214 - 226. [Abstract] [Full Text] [PDF] L. G. Chase, F. Ulloa-Montoya, B. L. Kidder, and C. M. Verfaillie Islet-Derived Fibroblast-Like Cells Are Not Derived via Epithelial-Mesenchymal Transition From Pdx-1 or Insulin-Positive Cells Diabetes, January 1, 2007; 56(1): 3 - 7. [Abstract] [Full Text] [PDF] G. C. Schatteman, M. Dunnwald, and C. Jiao Biology of bone marrow-derived endothelial cell precursors Am J Physiol Heart Circ Physiol, January 1, 2007; 292(1): H1 - H18. [Abstract] [Full Text] [PDF] X.-c. Chen, R. Wang, X. Zhao, Y.-q. Wei, M. Hu, Y.-s. Wang, X.-w. Zhang, R. Zhang, L. Zhang, B. Yao, et al. Prophylaxis against carcinogenesis in three kinds of unestablished tumor models via IL12-gene-engineered MSCs Carcinogenesis, December 1, 2006; 27(12): 2434 - 2441. [Abstract] [Full Text] [PDF] R. H. Lee, M. J. Seo, R. L. Reger, J. L. Spees, A. A. Pulin, S. D. Olson, and D. J. Prockop Multipotent stromal cells from human marrow home to and promote repair of pancreatic islets and renal glomeruli in diabetic NOD/scid mice PNAS, November 14, 2006; 103(46): 17438 - 17443. [Abstract] [Full Text] [PDF] M. D. Plotkin and M. S. Goligorsky Mesenchymal cells from adult kidney support angiogenesis and differentiate into multiple interstitial cell types including erythropoietin-producing fibroblasts Am J Physiol Renal Physiol, October 1, 2006; 291(4): F902 - F912. [Abstract] [Full Text] [PDF] A. J. Nauta, G. Westerhuis, A. B. Kruisselbrink, E. G. A. Lurvink, R. Willemze, and W. E. Fibbe Donor-derived mesenchymal stem cells are immunogenic in an allogeneic host and stimulate donor graft rejection in a nonmyeloablative setting Blood, September 15, 2006; 108(6): 2114 - 2120. [Abstract] [Full Text] [PDF] N. Riggi, L. Cironi, P. Provero, M.-L. Suva, J.-C. Stehle, K. Baumer, L. Guillou, and I. Stamenkovic Expression of the FUS-CHOP Fusion Protein in Primary Mesenchymal Progenitor Cells Gives Rise to a Model of Myxoid Liposarcoma. Cancer Res., July 15, 2006; 66(14): 7016 - 7023. [Abstract] [Full Text] [PDF] M. Sudres, F. Norol, A. Trenado, S. Gregoire, F. Charlotte, B. Levacher, J.-J. Lataillade, P. Bourin, X. Holy, J.-P. Vernant, et al. Bone Marrow Mesenchymal Stem Cells Suppress Lymphocyte Proliferation In Vitro but Fail to Prevent Graft-versus-Host Disease in Mice. J. Immunol., June 15, 2006; 176(12): 7761 - 7767. [Abstract] [Full Text] [PDF] B. T. Himes, B. Neuhuber, C. Coleman, R. Kushner, S. A. Swanger, G. C. Kopen, J. Wagner, J. S. Shumsky, and I. Fischer Recovery of Function Following Grafting of Human Bone Marrow-Derived Stromal Cells into the Injured Spinal Cord Neurorehabil Neural Repair, June 1, 2006; 20(2): 278 - 296. [Abstract] [PDF] J. Tolar, M. J. O'Shaughnessy, A. Panoskaltsis-Mortari, R. T. McElmurry, S. Bell, M. Riddle, R. S. McIvor, S. R. Yant, M. A. Kay, D. Krause, et al. Host factors that impact the biodistribution and persistence of multipotent adult progenitor cells Blood, May 15, 2006; 107(10): 4182 - 4188. [Abstract] [Full Text] [PDF] B. Gangadharan, E. T. Parker, L. M. Ide, H. T. Spencer, and C. B. Doering High-level expression of porcine factor VIII from genetically modified bone marrow-derived stem cells Blood, May 15, 2006; 107(10): 3859 - 3864. [Abstract] [Full Text] [PDF] N. Riggi, L. Cironi, P. Provero, M.-L. Suva, K. Kaloulis, C. Garcia-Echeverria, F. Hoffmann, A. Trumpp, and I. Stamenkovic Development of Ewing's Sarcoma from Primary Bone Marrow-Derived Mesenchymal Progenitor Cells Cancer Res., December 15, 2005; 65(24): 11459 - 11468. [Abstract] [Full Text] [PDF] H. Nakagami, K. Maeda, R. Morishita, S. Iguchi, T. Nishikawa, Y. Takami, Y. Kikuchi, Y. Saito, K. Tamai, T. Ogihara, et al. Novel Autologous Cell Therapy in Ischemic Limb Disease Through Growth Factor Secretion by Cultured Adipose Tissue-Derived Stromal Cells Arterioscler Thromb Vasc Biol, December 1, 2005; 25(12): 2542 - 2547. [Abstract] [Full Text] [PDF] A. Nakamizo, F. Marini, T. Amano, A. Khan, M. Studeny, J. Gumin, J. Chen, S. Hentschel, G. Vecil, J. Dembinski, et al. Human Bone Marrow-Derived Mesenchymal Stem Cells in the Treatment of Gliomas Cancer Res., April 15, 2005; 65(8): 3307 - 3318. [Abstract] [Full Text] [PDF] F. Anjos-Afonso, E. K. Siapati, and D. Bonnet In vivo contribution of murine mesenchymal stem cells into multiple cell-types under minimal damage conditions J. Cell Sci., November 1, 2004; 117(23): 5655 - 5664. [Abstract] [Full Text] [PDF] S. A. Rodeo, S. A. Maher, and C. Hidaka What's New in Orthopaedic Research J. Bone Joint Surg. Am., September 1, 2004; 86(9): 2085 - 2095. [Full Text] [PDF] M. Morigi, B. Imberti, C. Zoja, D. Corna, S. Tomasoni, M. Abbate, D. Rottoli, S. Angioletti, A. Benigni, N. Perico, et al. Mesenchymal Stem Cells Are Renotropic, Helping to Repair the Kidney and Improve Function in Acute Renal Failure J. Am. Soc. Nephrol., July 1, 2004; 15(7): 1794 - 1804. [Abstract] [Full Text] [PDF]

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