SEARCH:   Advanced

Blood, 15 May 2004, Vol. 103, No. 10, pp. 3805-3812. Prepublished online as a Blood First Edition Paper on December 30, 2003; DOI 10.1182/blood-2003-09-3109. This Article Full Text (PDF) All Versions of this Article: 2003-09-3109v1 103/10/3805    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ellyard, J. I Articles by Tangye, S. G Search for Related Content PubMed PubMed Citation Articles by Ellyard, J. I Articles by Tangye, S. G Social Bookmarking                   What's this? Submitted September 11, 2003 Accepted December 19, 2003 Ag-selected Ig-secreting cells persist in human spleen as well as bone marrow Julia I Ellyard, Danielle T Avery, Tri Giang Phan, Nathan J Hare, Philip D Hodgkin, and Stuart G Tangye* Centenary Institute of Cancer Medicine and Cell Biology, Newtown, NSW, Australia; University of Sydney, Sydney, NSW, Australia Centenary Institute of Cancer Medicine and Cell Biology, Newtown, NSW, Australia Walter and Eliza Hall Institute, Parkville, VIC, Australia * Corresponding author; email: s.tangye{at}centenary.usyd.edu.au. Plasma cells (PC) represent the final stage of B cell differentiation and are devoted to the production of Ig. Perturbations to their development can result in human disorders characterised by PC expansions and hypergammaglobulinaemia. Ig-secreting cells (ISC) have been identified in secondary lymphoid tissues and bone marrow (BM). The majority of ISC in lymphoid tissues are short-lived; in contrast, ISC that migrate to the BM become long-lived PC and continue to secrete Ig for extended periods. However, a small population of long-lived PC has been identified in rodent spleen suggesting PC may persist in secondary lymphoid tissues and that the spleen, as well as the BM, plays an important role in maintaining long-term humoral immunity. For these reasons, we examined ISC in human spleen and identified a population that appears analogous to long-lived rodent splenic PC. Human splenic ISC shared morphological, cellular, molecular and functional characteristics with long-lived PC in BM, demonstrating their commitment to the PC lineage. Furthermore, detection of highly mutated Ig V region genes in splenic ISC suggested they are likely to be Ag-selected and secrete high affinity Ig. Thus, our results suggest that splenic ISC have an important role in humoral immunity and may represent the affected cell type in some B-cell dyscrasias. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. Solomon, S. D. Macy, C. Wooliver, D. T. Weiss, and P. Westermark Splenic plasma cells can serve as a source of amyloidogenic light chains Blood, February 12, 2009; 113(7): 1501 - 1503. [Abstract] [Full Text] [PDF] K. L. Good, D. T. Avery, and S. G. Tangye Resting Human Memory B Cells Are Intrinsically Programmed for Enhanced Survival and Responsiveness to Diverse Stimuli Compared to Naive B Cells J. Immunol., January 15, 2009; 182(2): 890 - 901. [Abstract] [Full Text] [PDF] R. Kapur, S. Ebeling, and A. Hagenbeek B-cell involvement in chronic graft-versus-host disease Haematologica, November 1, 2008; 93(11): 1702 - 1711. [Abstract] [Full Text] [PDF] D. T. Avery, V. L. Bryant, C. S. Ma, R. de Waal Malefyt, and S. G. Tangye IL-21-Induced Isotype Switching to IgG and IgA by Human Naive B Cells Is Differentially Regulated by IL-4 J. Immunol., August 1, 2008; 181(3): 1767 - 1779. [Abstract] [Full Text] [PDF] I. Gonzalez-Garcia, B. Rodriguez-Bayona, F. Mora-Lopez, A. Campos-Caro, and J. A. Brieva Increased survival is a selective feature of human circulating antigen-induced plasma cells synthesizing high-affinity antibodies Blood, January 15, 2008; 111(2): 741 - 749. [Abstract] [Full Text] [PDF] V. L. Bryant, C. S. Ma, D. T. Avery, Y. Li, K. L. Good, L. M. Corcoran, R. de Waal Malefyt, and S. G. Tangye Cytokine-Mediated Regulation of Human B Cell Differentiation into Ig-Secreting Cells: Predominant Role of IL-21 Produced by CXCR5+ T Follicular Helper Cells J. Immunol., December 15, 2007; 179(12): 8180 - 8190. [Abstract] [Full Text] [PDF] F. Medina, C. Segundo, G. Jimenez-Gomez, I. Gonzalez-Garcia, A. Campos-Caro, and J. A. Brieva Higher maturity and connective tissue association distinguish resident from recently generated human tonsil plasma cells J. Leukoc. Biol., December 1, 2007; 82(6): 1430 - 1436. [Abstract] [Full Text] [PDF] D. Quandt, H. Hoff, M. Rudolph, S. Fillatreau, and M. C. Brunner-Weinzierl A New Role of CTLA-4 on B Cells in Thymus-Dependent Immune Responses In Vivo J. Immunol., December 1, 2007; 179(11): 7316 - 7324. [Abstract] [Full Text] [PDF] D. R. Withers, C. Fiorini, R. T. Fischer, R. Ettinger, P. E. Lipsky, and A. C. Grammer T cell dependent survival of CD20+ and CD20 plasma cells in human secondary lymphoid tissue Blood, June 1, 2007; 109(11): 4856 - 4864. [Abstract] [Full Text] [PDF] J. Wehner, C. N. Morrell, T. Reynolds, E. R. Rodriguez, and W. M. Baldwin III Antibody and Complement in Transplant Vasculopathy Circ. Res., February 2, 2007; 100(2): 191 - 203. [Abstract] [Full Text] [PDF] K. L. Good, V. L. Bryant, and S. G. Tangye Kinetics of Human B Cell Behavior and Amplification of Proliferative Responses following Stimulation with IL-21 J. Immunol., October 15, 2006; 177(8): 5236 - 5247. [Abstract] [Full Text] [PDF] V. Racanelli, M. A. Frassanito, P. Leone, M. Galiano, V. De Re, F. Silvestris, and F. Dammacco Antibody Production and In Vitro Behavior of CD27-Defined B-Cell Subsets: Persistent Hepatitis C Virus Infection Changes the Rules. J. Virol., April 1, 2006; 80(8): 3923 - 3934. [Abstract] [Full Text] [PDF] I. Gonzalez-Garcia, E. Ocana, G. Jimenez-Gomez, A. Campos-Caro, and J. A. Brieva Immunization-Induced Perturbation of Human Blood Plasma Cell Pool: Progressive Maturation, IL-6 Responsiveness, and High PRDI-BF1/BLIMP1 Expression Are Critical Distinctions between Antigen-Specific and Nonspecific Plasma Cells J. Immunol., April 1, 2006; 176(7): 4042 - 4050. [Abstract] [Full Text] [PDF] A. K. Cuss, D. T. Avery, J. L. Cannons, L. J. Yu, K. E. Nichols, P. J. Shaw, and S. G. Tangye Expansion of Functionally Immature Transitional B Cells Is Associated with Human-Immunodeficient States Characterized by Impaired Humoral Immunity J. Immunol., February 1, 2006; 176(3): 1506 - 1516. [Abstract] [Full Text] [PDF] G. R.A. Ehrhardt, J. T. Hsu, L. Gartland, C.-M. Leu, S. Zhang, R. S. Davis, and M. D. Cooper Expression of the immunoregulatory molecule FcRH4 defines a distinctive tissue-based population of memory B cells J. Exp. Med., September 19, 2005; 202(6): 783 - 791. [Abstract] [Full Text] [PDF] P. Zwollo, S. Cole, E. Bromage, and S. Kaattari B Cell Heterogeneity in the Teleost Kidney: Evidence for a Maturation Gradient from Anterior to Posterior Kidney J. Immunol., June 1, 2005; 174(11): 6608 - 6616. [Abstract] [Full Text] [PDF] D. T. Avery, J. I. Ellyard, F. Mackay, L. M. Corcoran, P. D. Hodgkin, and S. G. Tangye Increased Expression of CD27 on Activated Human Memory B Cells Correlates with Their Commitment to the Plasma Cell Lineage J. Immunol., April 1, 2005; 174(7): 4034 - 4042. [Abstract] [Full Text] [PDF]

	Copyright © 2003 by American Society of Hematology         Online ISSN: 1528-0020