SEARCH:   Advanced

Blood, 15 May 2004, Vol. 103, No. 10, pp. 3744-3750. Prepublished online as a Blood First Edition Paper on February 5, 2004; DOI 10.1182/blood-2003-09-3126. This Article Full Text (PDF) All Versions of this Article: 2003-09-3126v1 103/10/3744    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Peltola, K. J Articles by Koskinen, P. J Search for Related Content PubMed PubMed Citation Articles by Peltola, K. J Articles by Koskinen, P. J Social Bookmarking                   What's this? Submitted September 11, 2003 Accepted January 18, 2004 Pim-1 kinase inhibits STAT5-dependent transcription via its interactions with SOCS1 and SOCS3 Katriina J Peltola, Kirsi Paukku, Teija L Aho, Marja Ruuska, Olli Silvennoinen, and Paivi J Koskinen* Turku Centre for Biotechnology, University of Turku/Abo Akademi University, Turku, Finland; Turku Graduate School of Biomedical Sciences, Turku, Finland Department of Virology, Haartman Institute, Helsinki, Finland; Programme for Developmental and Reproductive Biology, Biomedicum Helsinki, Helsinki, Finland Turku Centre for Biotechnology, University of Turku/Abo Akademi University, Turku, Finland Programme for Developmental and Reproductive Biology, Biomedicum Helsinki, Helsinki, Finland; Institute of Medical Technology, University of Tampere, Tampere, Finland; Deaprtment of Clinical Microbiology, Tampere University Hospital, Tampere, Finland * Corresponding author; email: paivi.koskinen{at}btk.utu.fi. STAT5 plays a critical role in cytokine-induced survival of hematopoietic cells. One of the STAT5 target genes is pim-1, which encodes an oncogenic serine/threonine kinase. Here we demonstrate that Pim-1 inhibits STAT5-dependent transcription in cells responsive to interleukin-3, prolactin or erythropoietin. Ectopic expression of Pim-1 in cytokine-dependent FDCP1 myeloid cells results in reduced tyrosine phosphorylation and DNA-binding of STAT5, indicating that Pim-1 interferes already with the initial steps of STAT5 activation. However, the Pim-1 kinase does not directly phosphorylate or bind to STAT5. By contrast, Pim-1 interacts with the SOCS1 and SOCS3 suppressors of cytokine signaling and potentiates their inhibitory effects on STAT5, most likely via phosphorylation-mediated stabilization of the SOCS proteins. Thus, both Pim and SOCS family proteins may be components of a negative feedback mechanism that allows STAT5 to attenuate its own activity. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: T. Motiwala, S. Majumder, K. Ghoshal, H. Kutay, J. Datta, S. Roy, D. M. Lucas, and S. T. Jacob PTPROt Inactivates the Oncogenic Fusion Protein BCR/ABL and Suppresses Transformation of K562 Cells J. Biol. Chem., January 2, 2009; 284(1): 455 - 464. [Abstract] [Full Text] [PDF] Y. Zhang, Z. Wang, and N. S. Magnuson Pim-1 Kinase-Dependent Phosphorylation of p21Cip1/WAF1 Regulates Its Stability and Cellular Localization in H1299 Cells Mol. Cancer Res., September 1, 2007; 5(9): 909 - 922. [Abstract] [Full Text] [PDF] T L Cibull, T D Jones, L Li, J N Eble, L Ann Baldridge, S R Malott, Y Luo, and L Cheng Overexpression of Pim-1 during progression of prostatic adenocarcinoma. J. Clin. Pathol., March 1, 2006; 59(3): 285 - 288. [Abstract] [Full Text] [PDF] D. Ungureanu and O. Silvennoinen SLIM Trims STATs: Ubiquitin E3 Ligases Provide Insights for Specificity in the Regulation of Cytokine Signaling Sci. Signal., October 4, 2005; 2005(304): pe49 - pe49. [Abstract] [Full Text] [PDF] C. J. Fox, P. S. Hammerman, and C. B. Thompson The Pim kinases control rapamycin-resistant T cell survival and activation J. Exp. Med., January 18, 2005; 201(2): 259 - 266. [Abstract] [Full Text] [PDF]

	Copyright © 2004 by American Society of Hematology         Online ISSN: 1528-0020