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Blood, 15 May 2004, Vol. 103, No. 10, pp. 3677-3683. Prepublished online as a Blood First Edition Paper on January 15, 2004; DOI 10.1182/blood-2003-09-3198. This Article Full Text (PDF) All Versions of this Article: 2003-09-3198v1 103/10/3677    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Rajagopalan, S. Articles by Kaplan, M. J Search for Related Content PubMed PubMed Citation Articles by Rajagopalan, S. Articles by Kaplan, M. J Related Collections Related Article in Blood Online Social Bookmarking                   What's this? Submitted September 17, 2003 Accepted January 6, 2004 Endothelial Cell Apoptosis in Systemic Lupus Erythematosus: A Common Pathway for Abnormal Vascular Function and Thrombosis Propensity Sanjay Rajagopalan, Emily C Somers, Robert D Brook, Christine Kehrer, Dana Pfenninger, Emily Lewis, Anjan Chakrabarti, Bruce C Richardson, Eric Shelden, William J McCune, and Mariana J Kaplan* Internal Medicine, University of Michigan, Ann Arbor, MI, USA Cell and Developmental Biology, University of Michigan, Ann Arbor, MI, USA * Corresponding author; email: makaplan{at}umich.edu. Women with systemic lupus erythematosus (SLE) are at risk for premature athero-thrombosis independent of Framingham risk factors. We investigated if endothelial cell (EC) apoptosis predicts abnormal vasomotor tone and contributes to circulating tissue factor (TF) levels in this disease. Brachial artery flow mediated (FMD) and nitroglycerin mediated dilation were determined in women with SLE, healthy controls and subjects with coronary artery disease (CAD) (n=43/group). Quantification of circulating apoptotic ECs was performed by flow cytometry [CD146+ cells that stained for Annexin V (CD146AnnV+)] and immunofluorescent microscopy. Plasma TF was measured by ELISA. Compared to healthy controls or CAD, SLE patients had higher numbers of circulating CD146AnnV+ (10 ±3, 18 ±5, and 89 ±32 cells/ml, respectively, mean+ SEM; p<0.01). Increased CD146AnnV+ cells correlated strongly with abnormal vascular function (p = 0.037). After adjusting for known predictors of endothelial function, CD146AnnV+ was the only variable that predicted FMD (= -4.5, p<0.001). Increased CD146AnnV+ was strongly associated with elevated levels of circulating TF (r= 0.46, p=0.002). Circulating apoptotic ECs are elevated in young females with SLE and strongly correlate with markedly abnormal vascular function and elevated TF levels. Heightened endothelial apoptosis may represent an important mechanism for development of athero-thrombosis in SLE. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Endothelial apoptosis: the missing link between atherosclerosis and SLE? Tihomir Stefanec Blood 2004 103: 3608-3609. [Full Text] [PDF] This article has been cited by other articles: W. Zhao, S. G. Thacker, J. B. Hodgin, H. Zhang, J. H. Wang, J. L. Park, A. Randolph, E. C. Somers, S. Pennathur, M. Kretzler, et al. The Peroxisome Proliferator-Activated Receptor {gamma} Agonist Pioglitazone Improves Cardiometabolic Risk and Renal Inflammation in Murine Lupus J. Immunol., August 15, 2009; 183(4): 2729 - 2740. [Abstract] [Full Text] [PDF] M. Elshal, A. Abdelaziz, A. Abbas, K. Mahmoud, H. Fathy, S. E. Mongy, S. 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