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Blood, 1 July 2004, Vol. 104, No. 1, pp. 192-199. Prepublished online as a Blood First Edition Paper on March 9, 2004; DOI 10.1182/blood-2003-09-3211. This Article Full Text (PDF) All Versions of this Article: 2003-09-3211v1 104/1/192    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Palena, C. Articles by Tsang, K.-Y. Search for Related Content PubMed PubMed Citation Articles by Palena, C. Articles by Tsang, K.-Y. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted September 22, 2003 Accepted February 25, 2004 Human B cells that hyperexpress a triad of costimulatory molecules via avipox-vector infection: an alternative source of efficient antigen-presenting cells Claudia Palena, MingZhu Zhu, Jeffrey Schlom*, and Kwong-Yok Tsang Laboratory of Tumor Immunology and Biology, National Cancer Institute, Bethesda, MD, USA * Corresponding author; email: js141c{at}nih.gov. Dendritic cells (DCs) are the most potent of the antigen-presenting cells (APCs). Preparation of sufficient numbers of mature DCs, however, is both costly and time-consuming. We have examined here the possibility of using an alternative source of APCs that would be easier to obtain, would not require extensive culture, and thus would be more applicable to human immunotherapy protocols. We show here that freshly isolated human B cells can be efficiently infected by a replication-defective fowlpox recombinant vector, designated rF-TRICOM, to markedly increase surface expression of the human costimulatory molecule B7-1 and moderately increase expression of ICAM-1 and LFA-3. Peptide-pulsed rF-TRICOM-infected B cells were highly efficient in activating antigen-specific human T cells and shown to be superior to the use of CD40L in enhancing APC potency. Moreover, when infection of freshly isolated B cells with rF-TRICOM was combined with CD40L, a still further marked enhancement of the antigen-presenting potency was observed. Ex-vivo generated antigen-specific T cells activated in this manner might be applied to experimental protocols, or used for adoptive transfer in immunotherapy protocols. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C. Palena, K. A. Foon, D. Panicali, A. G. Yafal, J. Chinsangaram, J. W. Hodge, J. Schlom, and K. Y. Tsang Potential approach to immunotherapy of chronic lymphocytic leukemia (CLL): enhanced immunogenicity of CLL cells via infection with vectors encoding for multiple costimulatory molecules Blood, November 15, 2005; 106(10): 3515 - 3523. [Abstract] [Full Text] [PDF] S. Yang, K.-Y. Tsang, and J. Schlom Induction of Higher-Avidity Human CTLs by Vector-Mediated Enhanced Costimulation of Antigen-Presenting Cells Clin. Cancer Res., August 1, 2005; 11(15): 5603 - 5615. [Abstract] [Full Text] [PDF]

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