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Blood, 1 March 2004, Vol. 103, No. 5, pp. 1949-1954. Prepublished online as a Blood First Edition Paper on October 30, 2003; DOI 10.1182/blood-2003-09-3249. This Article Full Text (PDF) All Versions of this Article: 2003-09-3249v1 103/5/1949    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ito, H. Articles by Sykes, M. Search for Related Content PubMed PubMed Citation Articles by Ito, H. Articles by Sykes, M. Related Collections Related Letter in Blood Online Social Bookmarking                   What's this? Submitted September 23, 2003 Accepted October 23, 2003 Local irradiation enhances congenic donor pluripotent hematopoietic stem cell engraftment similarly in irradiated and non-irradiated sites Hiroshi Ito, Yasuo Takeuchi, Juanita Shaffer, and Megan Sykes* Transplantation Biology Research Center/Bone Marrow Transplantation Section, Massachusetts General Hospital/Harvard Medical School, Boston, MA, USA * Corresponding author; email: megan.sykes{at}tbrc.mgh.harvard.edu. Long-term multilineage chimerism is achieved in CD45 congenic mice receiving high bone marrow doses with or without mediastinal irradiation (MI). Increased donor chimerism results in MI-treated compared to unirradiated animals, suggesting that MI makes "space" for engraftment of donor pluripotent hematopoietic stem cells (PHSC). We have now examined whether "space" is systemic, or whether increased engraftment of donor marrow in locally irradiated mice is confined to the irradiated bones. While increased donor chimerism was observed in irradiated bones compared to non-irradiated bones of MI-treated animals 4 weeks following BMT, these differences were minimal by 40 weeks. MI-treated chimeras contained more adoptively transferable donor PHSC in the marrow of both irradiated and distant bones compared to non-MI treated chimeras. Similar proportions of donor PHSC were present in irradiated and non-irradiated bones of locally irradiated mice at both 4 and 40 weeks. Irradiated bones contained more of donor short-term repopulating cells than distant bones at 4 weeks, but not 40 weeks, post-BMT. Our study suggests that local proliferation of donor PHSC in mice receiving local irradiation rapidly leads to a systemic increase in donor PHSC engraftment. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Letter in Blood Online: The balance among immune suppression, stem cell ablation, competition, and engraftment Ronald van Os, Gerald de Haan, Hiroshi Ito, and Megan Sykes Blood 2004 103: 2859. [Full Text] [PDF] This article has been cited by other articles: H. Xu, J. Yan, Y. Huang, P. M. Chilton, C. Ding, C. L. Schanie, L. Wang, and S. T. Ildstad Costimulatory blockade of CD154-CD40 in combination with T-cell lymphodepletion results in prevention of allogeneic sensitization Blood, March 15, 2008; 111(6): 3266 - 3275. [Abstract] [Full Text] [PDF] C. Couldrey, H. L. Bradley, and K. D. Bunting A STAT5 modifier locus on murine chromosome 7 modulates engraftment of hematopoietic stem cells during steady-state hematopoiesis Blood, February 15, 2005; 105(4): 1476 - 1483. [Abstract] [Full Text] [PDF] H. Xu, B. G. Exner, P. M. Chilton, C. Schanie, and S. T. Ildstad CD45 Congenic Bone Marrow Transplantation: Evidence for T Cell-Mediated Immunity Stem Cells, November 1, 2004; 22(6): 1039 - 1048. [Abstract] [Full Text] [PDF] R. van Os, G. de Haan, H. Ito, and M. Sykes The balance among immune suppression, stem cell ablation, competition, and engraftment Blood, April 1, 2004; 103(7): 2859 - 2859. [Full Text] [PDF]

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