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Blood, 15 May 2004, Vol. 103, No. 10, pp. 3624-3634.
Prepublished online as a Blood First Edition Paper on January 15, 2004; DOI 10.1182/blood-2003-09-3295.
Submitted October 6, 2003
Accepted December 4, 2003
Proteomics techniques and their application to hematology
Ileana M Cristea, Simon J Gaskell, and Anthony D Whetton*
Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, NY, USA; Michael Barber Centre for Mass Spectrometry, Dept of Chemistry, UMIST, Manchester, United Kingdom
Michael Barber Centre for Mass Spectrometry, Dept of Chemistry, UMIST, Manchester, United Kingdom
Paterson Institute for Cancer Research, Christie Hospital, Manchester, United Kingdom; Leukaemia Research Fund Proteomics Facility, Dept of Biomolecular Sciences, UMIST, Manchester, United Kingdom
* Corresponding author; email: tony.whetton{at}umist.ac.uk.
The recent sequencing of a number of genomes has raised the level of opportunities for studies on proteins. This area of research has been described with the all-embracing term, proteomics. In proteomics, the use of mass spectrometric techniques enables genomic databases to be employed to establish the identity of proteins with relatively little data compared to the pre-genome era. The use of related analytical techniques also offers the opportunity to gain information on regulation, via post-translational modification, and potential new diagnostic and prognostic indicators. Relative quantification of proteins and peptides in cellular and extracellular material remains a challenge for proteomics and mass spectrometry. This review presents an analysis of the present and future impact of these proteomic technologies with emphasis on relative quantification for hematological research giving an appraisal of their potential benefits.
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