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Blood, 15 August 2004, Vol. 104, No. 4, pp. 1025-1033. Prepublished online as a Blood First Edition Paper on May 4, 2004; DOI 10.1182/blood-2003-09-3334. This Article Full Text (PDF) All Versions of this Article: 2003-09-3334v1 104/4/1025    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Yuan, K. Articles by Lin, M. T Search for Related Content PubMed PubMed Citation Articles by Yuan, K. Articles by Lin, M. T Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted October 2, 2003 Accepted March 23, 2004 Syndecan-1 upregulated by ephrinB2/EphB4 plays dual roles in inflammatory angiogenesis Kuo Yuan, Tse-Ming Hong, Jeremy Chen, Wan-Hua Tsai, and Ming T Lin* Periodontics Division, Department of Dentistry, National Cheng Kung University Hospital, Tainan City, Taiwan; Department of Biochemistry and Institute of Basic Medical Sciences, Medical School, National Cheng Kung University, Tainan City, Taiwan School of Pharmacy, Natinal Taiwan University College of Medicine, Taipei City, Taiwan Institute of Biomedical Sciences and Molecular Biology, National Chung Hsing University, Taichung, Taiwan Department of Biochemistry and Institute of Basic Medical Sciences, Medical School, National Cheng Kung University, Tainan City, Taiwan * Corresponding author; email: Lin1223{at}mail.ncku.edu.tw. EphrinB2 and EphB4, its cognate receptor, are important in the vascular development of the mouse embryo. Their roles in human inflammatory angiogenesis, however, are not well understood. By examining hyper-inflammatory lesions, we saw that ephrinB2 was predominantly expressed in macrophage-like cells, and EphB4, in small venules. Because macrophages usually transmigrate through post-capillary venules during inflammation, we wanted to explore the downstream effects of EphB4 after binding to ephrinB2. By using cDNA microarray technique and following RT-PCR, we found that syntenin and syndecan-1 were up-regulated in EphB4+ endothelial cells dose- and time-dependently after stimulation with pre-clustered ephrinB2. In vitro, ephrinB2 suppressed the angiogenic effects of bFGF on EphB4+ endothelial cells, partially due to syndecan-1's competition with FGFR for bFGF. However, ephrinB2 exhibited angiogenic effects in vivo, possibly due to an inflammation-associated enzyme-heparanase. The enzymes could convert the inhibitory effect of ephrinB2 on EphB4+ endothelial cells to an activating effect by removing poorly sulfated side chains of upregulated syndecan-1 ectodomain. Depending on the presence of heparanases, the roles of syndecan-1 may be opposite in different physiological settings. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C. Cappuzzello, R. Melchionna, A. Mangoni, G. Tripodi, P. Ferrari, L. Torielli, D. Arcelli, M. Helmer-Citterich, G. Bianchi, M. C. Capogrossi, et al. Role of rat {alpha} adducin in angiogenesis: Null effect of the F316Y polymorphism Cardiovasc Res, August 1, 2007; 75(3): 608 - 617. [Abstract] [Full Text] [PDF] S. Mitola, M. Belleri, C. Urbinati, D. Coltrini, B. Sparatore, M. Pedrazzi, E. Melloni, and M. Presta Cutting Edge: Extracellular High Mobility Group Box-1 Protein Is a Proangiogenic Cytokine J. Immunol., January 1, 2006; 176(1): 12 - 15. [Abstract] [Full Text] [PDF] E. Tkachenko, J. M. Rhodes, and M. Simons Syndecans: New Kids on the Signaling Block Circ. Res., March 18, 2005; 96(5): 488 - 500. [Abstract] [Full Text] [PDF]

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