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Blood, 1 June 2004, Vol. 103, No. 11, pp. 4310-4316. Prepublished online as a Blood First Edition Paper on February 19, 2004; DOI 10.1182/blood-2003-09-3362. This Article Full Text (PDF) All Versions of this Article: 2003-09-3362v1 103/11/4310    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Carlile, G. W Articles by Wiedmann, M. Search for Related Content PubMed PubMed Citation Articles by Carlile, G. W Articles by Wiedmann, M. Social Bookmarking                   What's this? Submitted September 30, 2003 Accepted February 8, 2004 Caspase-3 has a Non-Apoptotic Function in Erythroid Maturation Graeme W Carlile, Deborah H Smith, and Martin Wiedmann* Cell Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY, USA; Weill Graduate School of Medical Sciences of Cornell University, New York, NY, USA Cell Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY, USA * Corresponding author; email: m-wiedmann{at}ski.mskcc.org. Caspase-3 plays a central role in apoptosis. It is also activated in normal erythropoiesis, with its activity peaking early during development (CFU-E stage). In the present study, we have reduced the expression and subsequent enzymatic activity of caspase-3 by transfection of small interfering RNA (siRNA) directed to caspase-3 in a differentiating human erythroid culture system. We find that siRNA treatment yields a 50% reduction in cells that undergo enucleation with no change in the fraction of cells that undergo apoptosis, measured throughout the culture. Furthermore, a substantial fraction of treated cells are unable to complete the transition from pronormoblasts to basophilic normoblasts. These results demonstrate that caspase-3 is required for efficient erythropoiesis in this model system. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. J. Weiss and C. O. dos Santos Chaperoning erythropoiesis Blood, March 5, 2009; 113(10): 2136 - 2144. [Abstract] [Full Text] [PDF] L. Blanc, C. Barres, P. Bette-Bobillo, and M. Vidal Reticulocyte-secreted exosomes bind natural IgM antibodies: involvement of a ROS-activatable endosomal phospholipase iPLA2 Blood, November 1, 2007; 110(9): 3407 - 3416. [Abstract] [Full Text] [PDF] L. De Franceschi, L. Ronzoni, M. D. Cappellini, F. 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