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Blood, 1 September 2004, Vol. 104, No. 5, pp. 1498-1503. Prepublished online as a Blood First Edition Paper on May 13, 2004; DOI 10.1182/blood-2003-10-3404. This Article Full Text (PDF) Supplemental Figures All Versions of this Article: 2003-10-3404v1 104/5/1498    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Chou, C.-F. Articles by Venkatesh, B. Search for Related Content PubMed PubMed Citation Articles by Chou, C.-F. Articles by Venkatesh, B. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted October 3, 2003 Accepted March 29, 2004 Erythropoietin gene from a teleost fish, Fugu rubripes Chih-Fong Chou, Sumanty Tohari, Sydney Brenner, and Byrappa Venkatesh* Molecular Genetics Lab, Institute of Molecular and Cell Biology, Singapore * Corresponding author; email: mcbbv{at}imcb.a-star.edu.sg. In this paper we report the cloning and characterization of erythropoietin (Epo) gene from the pufferfish, Fugu rubripes. This is the first non-mammalian Epo gene to be cloned. The Fugu Epo comprises five exons and four introns similar to the human EPO, and encodes a 185-amino acid protein that is 32% to 34% identical to Epo from various mammals. The synteny of genes at the Epo locus is conserved between the Fugu and humans. Unlike in mammals in which adult kidney is the primary Epo-producing organ, the heart is the main Epo-producing organ in adult Fugu. In addition to the heart, Fugu Epo is also expressed in the liver and brain similar to the human EPO. Interestingly, the transcripts in the Fugu brain are generated from a distal promoter and include an alternatively spliced first coding exon. No such brain-specific alternative splicing of Epo has been reported in mammals so far. Transient transfection studies in a fish hepatoma cell line (PLHC-1) and a human hepatoma cell line (HepG2) suggest that although Fugu Epo promoter many not be hypoxia inducible, the gene may be regulated by hypoxia. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Is there something fishy about EPO? Constance T. Noguchi Blood 2004 104: 1238. [Full Text] [PDF] This article has been cited by other articles: N. Paffett-Lugassy, N. Hsia, P. G. Fraenkel, B. Paw, I. Leshinsky, B. Barut, N. Bahary, J. Caro, R. Handin, and L. I. Zon Functional conservation of erythropoietin signaling in zebrafish Blood, October 1, 2007; 110(7): 2718 - 2726. [Abstract] [Full Text] [PDF] T. van der Meulen, H. Schipper, J. G. M. van den Boogaart, M. O. Huising, S. Kranenbarg, and J. L. van Leeuwen Endurance exercise differentially stimulates heart and axial muscle development in zebrafish (Danio rerio). Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2006; 291(4): R1040 - R1048. [Abstract] [Full Text] [PDF] M. O Huising, C. P Kruiswijk, and G. Flik Phylogeny and evolution of class-I helical cytokines. J. Endocrinol., April 1, 2006; 189(1): 1 - 25. [Abstract] [Full Text] [PDF] Y. Aizawa, N. Nogawa, N. Kosaka, Y. Maeda, T. Watanabe, H. Miyazaki, and T. Kato Expression of Erythropoietin Receptor-Like Molecule in Xenopus laevis and Erythrocytopenia upon Administration of Its Recombinant Soluble Form J. Biochem., August 1, 2005; 138(2): 167 - 175. [Abstract] [Full Text] [PDF] M. Nikinmaa and B. B. Rees Oxygen-dependent gene expression in fishes Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2005; 288(5): R1079 - R1090. [Abstract] [Full Text] [PDF]

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