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Blood, 1 August 2004, Vol. 104, No. 3, pp. 675-686. Prepublished online as a Blood First Edition Paper on April 13, 2004; DOI 10.1182/blood-2003-10-3423. This Article Full Text (PDF) All Versions of this Article: 2003-10-3423v1 104/3/675    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Wagner, W. Articles by Ho, A. D Search for Related Content PubMed PubMed Citation Articles by Wagner, W. Articles by Ho, A. D Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted October 7, 2003 Accepted March 24, 2004 Molecular evidence for stem cell function of the slow dividing fraction among human hematopoietic progenitor cells by genome wide analysis Wolfgang Wagner, Alexandra Ansorge, Ute Wirkner, Volker Eckstein, Christian Schwager, Jonathon Blake, Katrin Miesala, Jan Selig, Rainer Saffrich, Wilhelm Ansorge, and Anthony D Ho* Medizinische Klinik und Poliklinik V, University of Heidelberg, Heidelberg, Germany Functional Genomics Analysis, Biochemical Instrumentation Programme, European Molecular Biology Laboratory, Heidelberg, Germany * Corresponding author; email: anthony_dick.ho{at}urz.uni-heidelberg.de. The molecular mechanisms that regulate asymmetric divisions of hematopoietic progenitor cells (HPC) are not yet understood. The slow dividing fraction (SDF) of HPC is associated with primitive function and self-renewal while the fast dividing fraction (FDF) predominantly proceeds to differentiation. CD34+/CD38- cells of human umbilical cord blood were separated into SDF and FDF. Genome wide gene expression analysis of these populations was determined using the newly developed Human Transcriptome Microarray containing 51.145 cDNA clones of the Unigene Set-RZPD3. In addition, gene expression profiles of CD34+/CD38- cells were compared with those of CD34+/CD38+ cells. Among the genes showing the highest expression levels in the SDF were the following: CD133, erg, cyclin g2, MDR1, osteopontin, clqr1, ifi16, jak3, fzd6 and hoxa9, a pattern compatible with their primitive function and self-renewal capacity. Furthermore morphologic differences between SDF and FDF were determined. Cells in the SDF have more membrane protrusions and CD133 is located on these lamellipodia. The majority of cells in the SDF are rhodamine-123dull. These results provide molecular evidence that the SDF is associated with primitive function and serves as basis for a detailed understanding of asymmetric division of stem cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Stem cells and the Heisenberg uncertainty principle H. Jeffrey Lawrence Blood 2004 104: 597-598. [Full Text] [PDF] This article has been cited by other articles: F. P. G. Silva, S. M. A. Swagemakers, C. Erpelinck-Verschueren, B. J. Wouters, R. Delwel, H. Vrieling, P. van der Spek, P. J. M. Valk, and M. Giphart-Gassler Gene expression profiling of minimally differentiated acute myeloid leukemia: M0 is a distinct entity subdivided by RUNX1 mutation status Blood, October 1, 2009; 114(14): 3001 - 3007. [Abstract] [Full Text] [PDF] A. Kandilci and G. C. 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Young Aberrant chromatin at genes encoding stem cell regulators in human mixed-lineage leukemia Genes & Dev., December 15, 2008; 22(24): 3403 - 3408. [Abstract] [Full Text] [PDF] M. A. Meester-Smoor, M. J.F.W. Janssen, G. C. Grosveld, A. de Klein, W. F.J. van IJcken, H. Douben, and E. C. Zwarthoff MN1 affects expression of genes involved in hematopoiesis and can enhance as well as inhibit RAR/RXR-induced gene expression Carcinogenesis, October 1, 2008; 29(10): 2025 - 2034. [Abstract] [Full Text] [PDF] C. Langer, M. D. Radmacher, A. S. Ruppert, S. P. Whitman, P. Paschka, K. Mrozek, C. D. Baldus, T. Vukosavljevic, C.-G. Liu, M. E. Ross, et al. High BAALC expression associates with other molecular prognostic markers, poor outcome, and a distinct gene-expression signature in cytogenetically normal patients younger than 60 years with acute myeloid leukemia: a Cancer and Leukemia Group B (CALGB) study Blood, June 1, 2008; 111(11): 5371 - 5379. [Abstract] [Full Text] [PDF] H. Araki, K. 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