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Blood, 1 June 2004, Vol. 103, No. 11, pp. 4134-4141. Prepublished online as a Blood First Edition Paper on February 12, 2004; DOI 10.1182/blood-2003-10-3575. This Article Full Text (PDF) Supplemental Data Set All Versions of this Article: 2003-10-3575v1 103/11/4134    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Lu, S.-J. Articles by Honig, G. R Search for Related Content PubMed PubMed Citation Articles by Lu, S.-J. Articles by Honig, G. R Social Bookmarking                   What's this? Submitted October 20, 2003 Accepted February 4, 2004 CD34CD38 Hematopoietic Precursors Derived from Human Embryonic Stem Cells Exhibit an Embryonic Gene Expression Pattern Shi-Jiang Lu*, Fei Li, Loyda Vida, and George R Honig Pediatrics, University of Illinois at Chicago, Chicago, IL, USA * Corresponding author; email: sjlu{at}uic.edu. Gene expression patterns of CD34+CD38- cells derived from human embryonic stem cells (ESC) were compared with those of cells isolated from adult human bone marrow (BM) using microarrays; 1,692 and 1,494 genes were expressed at levels 3 fold above background in cells from BM and ESC, respectively. Of these, 494 showed similar levels of expression in cells from both sources, 791 genes were overexpressed in cells from BM (BM vs. ESC 2-fold), and 803 genes were preferentially expressed in cells from ESC (ESC vs. BM 2-fold). The message of the flt-3 gene was markedly decreased in cells from ESC, whereas there was substantial flt-3 expression in cells from BM. High levels of embryonic -globin expression were observed, but no adult -globin message, in CD34+CD38- cells from ESC, whereas high levels of -globin expression, but no embryonic -globin message, could be detected in cells from BM. Furthermore, high levels of MHC gene expression were demonstrated in cells from BM, but very low levels of MHC message in corresponding cells from ESC. These observations demonstrate that CD34+CD38- cells derived from ESC correspond consistently to an early developmental stage at which the yolk sac and fetal liver are the primary sites of hematopoiesis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S.-J. Lu, Q. Feng, J. S. Park, L. Vida, B.-S. Lee, M. Strausbauch, P. J. Wettstein, G. R. Honig, and R. 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