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Blood, 1 November 2004, Vol. 104, No. 9, pp. 2761-2766. Prepublished online as a Blood First Edition Paper on July 8, 2004; DOI 10.1182/blood-2003-10-3614. This Article Full Text (PDF) All Versions of this Article: 2003-10-3614v1 104/9/2761    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Hristov, M. Articles by Weber, P. C Search for Related Content PubMed PubMed Citation Articles by Hristov, M. Articles by Weber, P. C Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted October 23, 2003 Accepted June 10, 2004 Apoptotic Bodies from Endothelial Cells Enhance the Number and Initiate the Differentiation of Human Endothelial Progenitor Cells in vitro Mihail Hristov*, Wolfgang Erl, Stefan Linder, and Peter C Weber Institute for Prevention of Cardiovascular Diseases, Ludwig-Maximilians-Universitat, Munich, Germany * Corresponding author; email: mhristov{at}med.uni-muenchen.de. Endothelial progenitor cells (EPC) play a role in the repair of ischemic or injured tissue. Because endothelial injury can be associated with apoptosis, we have investigated whether apoptotic bodies from mature endothelial cells (EC) may affect EPC growth and differentiation in vitro. A 24 h incubation of isolated human EPC with apoptotic bodies-rich medium (ABRM) from EC lead to a significant increase in the number of spindle-shaped attached cells. EPC were characterized by DiI-Ac-LDL/lectin staining and measurement of CD34 and kinase insert domain receptor (KDR) expression. The treatment with ABRM resulted in a twofold increase of DiI-Ac-LDL/lectin positive cells and upregulation of CD34 (22.2 ± 1.8% vs. 13.1 ± 2.7%, P< 0.05) and KDR (49.4 ± 12.3% vs. 18.8 ± 7.1%, P< 0.05). Fluorescence and confocal laser microscopy demonstrated the uptake of apoptotic bodies by the EPC. Apoptotic bodies-depleted medium had no effect, whereas the incubation with suspension of apoptotic bodies induced effects similar to those of ABRM. Our results suggest that apoptotic bodies from EC are taken up by EPC, increasing their number and differentiation state. Such a mechanism may facilitate the repair of injured endothelium and may represent a new signaling pathway between progenitor and damaged somatic cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: The endothelial life insurance plan David C. Sane Blood 2004 104: 2615-2616. [Full Text] [PDF] This article has been cited by other articles: A. D. Bhatwadekar, J. V. Glenn, T. M. Curtis, M. B. Grant, A. W. Stitt, and T. A. Gardiner Retinal Endothelial Cell Apoptosis Stimulates Recruitment of Endothelial Progenitor Cells Invest. Ophthalmol. Vis. Sci., October 1, 2009; 50(10): 4967 - 4973. [Abstract] [Full Text] [PDF] I. Dursun, H. M Poyrazoglu, Z. Gunduz, H. Ulger, A. Yykylmaz, R. Dusunsel, T. Patyroglu, and M. Gurgoze The relationship between circulating endothelial microparticles and arterial stiffness and atherosclerosis in children with chronic kidney disease Nephrol. Dial. 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