|
|
Blood, 15 December 2004, Vol. 104, No. 13, pp. 3911-3917.
Prepublished online as a Blood First Edition Paper on August 12, 2004; DOI 10.1182/blood-2003-10-3688.
 Previous Article | Next Article 
Submitted October 29, 2003
Accepted July 28, 2004
Expression of C/EBP Homologous Protein (CHOP) is Regulated by Retinoic Acid, and CHOP Negatively Regulates Myeloid Target Genes
Sigal Gery*, Dorothy J Park, Peter T Vuong, Doris Y Chih, Nathan Lemp, and H P Koeffler
Division of Hematology/Oncology, Cedars-Sinai Medical Center, UCLA School of Medicine, Los Angeles, CA, USA
* Corresponding author; email: gerys{at}cshs.org.
Retinoic acid (RA) promotes granulocytic differentiation of normal hematopoietic cells as well as acute promyelocytic leukemia (APL) blasts by transcriptional modulation of myeloid regulatory genes. In this study, we have identified the C/EBP Homologous Protein (CHOP) as a novel retinoid responsive gene using a PCR-based cDNA subtraction method. All-trans retinoic acid (ATRA) induced a biphasic expression of CHOP mRNA in the NB4 and HL60 AML cell lines. Level of CHOP expression increased within 1 hour of exposure to ATRA, its expression became nearly absent between 6 to 24 hours, and a second phase of induction occurred after 48 hours. Retinoid-dependent regulation of CHOP expression was also observed in normal human neutrophils, but not in peripheral blood mononuclear cells. In addition, retinoid-dependent regulation of CHOP expression was not observed in the retinoid non-responsive cell lines HL60R and NB4-R2. CHOP expression was regulated at the transcriptional level and was independent of new protein synthesis. CHOP heterodimerized with C/EBP and negatively regulated the myeloid specific gene, lactoferrin. Furthermore, CHOP transcriptionally inhibited C/EBP and --dependent induction of secondary granule gene expression. Taken together, RA signaling in granulocytic differentiation involves regulated expression of CHOP and C/EBP in a coordinated fashion.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
S. Doulatov, F. Notta, K. L. Rice, L. Howell, A. Zelent, J. D. Licht, and J. E. Dick
PLZF is a regulator of homeostatic and cytokine-induced myeloid development
Genes & Dev.,
September 1, 2009;
23(17):
2076 - 2087.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Pendino, E. Nguyen, I. Jonassen, B. Dysvik, A. Azouz, M. Lanotte, E. Segal-Bendirdjian, and J. R. Lillehaug
Functional involvement of RINF, retinoid-inducible nuclear factor (CXXC5), in normal and tumoral human myelopoiesis
Blood,
April 2, 2009;
113(14):
3172 - 3181.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Wei, S. Puzhko, M. Wabitsch, and C. G. Goodyer
Transcriptional Regulation of the Human Growth Hormone Receptor (hGHR) Gene V2 Promoter by Transcriptional Activators and Repressor
Mol. Endocrinol.,
March 1, 2009;
23(3):
373 - 387.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Wiper-Bergeron, C. St-Louis, and J. M. Lee
CCAAT/Enhancer Binding Protein {beta} Abrogates Retinoic Acid-Induced Osteoblast Differentiation via Repression of Runx2 Transcription
Mol. Endocrinol.,
September 1, 2007;
21(9):
2124 - 2135.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M. Chumakov, A. Silla, E. A. Williamson, and H. P. Koeffler
Modulation of DNA binding properties of CCAAT/enhancer binding protein epsilon by heterodimer formation and interactions with NFkappaB pathway
Blood,
May 15, 2007;
109(10):
4209 - 4219.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Takeda, C. Goolsby, and N. R. Yaseen
NUP98-HOXA9 Induces Long-term Proliferation and Blocks Differentiation of Primary Human CD34+ Hematopoietic Cells.
Cancer Res.,
July 1, 2006;
66(13):
6628 - 6637.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
