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Blood, 15 August 2004, Vol. 104, No. 4, pp. 993-1001. Prepublished online as a Blood First Edition Paper on April 20, 2004; DOI 10.1182/blood-2003-10-3702. This Article Full Text (PDF) All Versions of this Article: 2003-10-3702v1 104/4/993    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Hidalgo, A. Articles by Frenette, P. S Search for Related Content PubMed PubMed Citation Articles by Hidalgo, A. Articles by Frenette, P. S Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted October 29, 2003 Accepted April 5, 2004 The integrin M2 anchors hematopoietic progenitors in the bone marrow during enforced mobilization Andres Hidalgo, Anna J Peired, Linnea A Weiss, Yoshio Katayama, and Paul S Frenette* Medicine and Immunobiology Center, Mount Sinai School of Medicine, New York, NY, USA * Corresponding author; email: paul.frenette{at}mssm.edu. The sulfated polysaccharide fucoidan can rapidly mobilize hematopoietic progenitor cells (HPCs) and long-term repopulating stem cells from the bone marrow (BM) to the circulation. While searching for mechanisms involved in this phenomenon, we found that BM myeloid cells bound to fucoidan through the integrin M2 (Mac-1) and L-selectin, resulting in M2-independent release of neutrophil elastase, but inhibition of elastase activity did not impair fucoidan-induced mobilization. Mobilization of HPCs by fucoidan was enhanced in animals deficient in M (M-/-) compared to wild-type (M+/+) animals and higher plasma levels of the chemokine CXCL12/SDF-1 were achieved in M-/- mice by fucoidan treatment. However, in chimeric animals harboring M+/+ and M-/- HPCs in the BM, M-/- HPCs were preferentially mobilized by fucoidan, suggesting that the enhanced mobilization is cell-intrinsic and does not result not from altered microenvironment. Suboptimal doses of G-CSF or cyclophosphamide (CY) also resulted in enhanced HPC mobilization in M-/- mice compared to M+/+ controls, but this difference was overcome when standard doses of G-CSF or CY were administered. Taken together, these data suggest that the integrin M2 participates in the retention of HPCs in the BM. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: H. Qian, K. Tryggvason, S. E. Jacobsen, and M. Ekblom Contribution of {alpha}6 integrins to hematopoietic stem and progenitor cell homing to bone marrow and collaboration with {alpha}4 integrins Blood, May 1, 2006; 107(9): 3503 - 3510. [Abstract] [Full Text] [PDF] D. E. Cramer, D. J. Allendorf, J. T. Baran, R. Hansen, J. Marroquin, B. Li, J. Ratajczak, M. Z. Ratajczak, and J. Yan {beta}-Glucan enhances complement-mediated hematopoietic recovery after bone marrow injury Blood, January 15, 2006; 107(2): 835 - 840. [Abstract] [Full Text] [PDF] T. Okazaki, S. Ebihara, M. Asada, A. Kanda, H. Sasaki, and M. Yamaya Granulocyte colony-stimulating factor promotes tumor angiogenesis via increasing circulating endothelial progenitor cells and Gr1+CD11b+ cells in cancer animal models Int. Immunol., January 1, 2006; 18(1): 1 - 9. [Abstract] [Full Text] [PDF] M. Pihlgren, M. Friedli, C. Tougne, A.-F. Rochat, P.-H. Lambert, and C.-A. Siegrist Reduced Ability of Neonatal and Early-Life Bone Marrow Stromal Cells to Support Plasmablast Survival J. Immunol., January 1, 2006; 176(1): 165 - 172. [Abstract] [Full Text] [PDF] P. C. E. Burdon, C. Martin, and S. M. Rankin The CXC chemokine MIP-2 stimulates neutrophil mobilization from the rat bone marrow in a CD49d-dependent manner Blood, March 15, 2005; 105(6): 2543 - 2548. [Abstract] [Full Text] [PDF]

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