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Blood, 15 June 2004, Vol. 103, No. 12, pp. 4432-4439. Prepublished online as a Blood First Edition Paper on February 26, 2004; DOI 10.1182/blood-2003-10-3705. This Article Full Text (PDF) Supplemental Table and Figure All Versions of this Article: 2003-10-3705v1 103/12/4432    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Richard, R. E Articles by Blau, C A Search for Related Content PubMed PubMed Citation Articles by Richard, R. E Articles by Blau, C A Social Bookmarking                   What's this? Submitted October 30, 2003 Accepted January 27, 2004 Modulating erythrocyte chimerism in a mouse model of pyruvate kinase deficiency Robert E Richard*, Michael Weinreich, Kai-Hsin Chang, Jessica Ieremia, Mary M Stevenson, and C A Blau Hematology/Medicine, University of Washington, Seattle, WA, USA Medicine, Montreal General Hospital Research Institute, Montreal, Quebec, Canada * Corresponding author; email: rrichard{at}u.washington.edu. In vivo selection may provide a means to increase the relative number of cells of donor origin in the setting of hemopoietic chimerism. We have tested whether in vivo selection using chemical inducers of dimerization (CID) can direct the expansion of transduced normal donor erythrocytes in the setting of chimerism using a mouse model of pyruvate kinase deficiency. Marrow cells from normal CBA/N mice were transduced with a vector (F36Vmpl) that promotes cell growth in the presence of CID. Transduced cells were then transplanted into minimally conditioned, pyruvate kinase deficient, recipients (CBA-Pk-1slc/Pk-1slc) to establish stable chimerism. CID administration resulted in expansion of normal donor erythrocytes and improvement of the anemia. The preferential expansion of normal erythrocytes also resulted in a decrease in erythropoietin levels, reducing the drive for production of pyruvate kinase deficient red cells. CID-mediated expansion of genetically modified erythrocytes could prove a useful adjunct to transplant methods that achieve erythroid chimerism. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. A. Weinreich, I. Lintmaer, L. Wang, H. D. Liggitt, M. A. Harkey, and C. A. Blau Growth factor receptors as regulators of hematopoiesis Blood, December 1, 2006; 108(12): 3713 - 3721. [Abstract] [Full Text] [PDF]

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