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Blood, 1 June 2004, Vol. 103, No. 11, pp. 4028-4035. Prepublished online as a Blood First Edition Paper on February 19, 2004; DOI 10.1182/blood-2003-11-3929. This Article Full Text (PDF) All Versions of this Article: 2003-11-3929v1 103/11/4028    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by MIESCHER, S. Articles by BICHLER, J. Search for Related Content PubMed PubMed Citation Articles by MIESCHER, S. Articles by BICHLER, J. Related Collections Related Letter in Blood Online Social Bookmarking                   What's this? Submitted November 17, 2003 Accepted February 2, 2004 A single recombinant anti-RhD IgG prevents Rhesus D immunization: association of RhD positive red blood cell clearance rate with polymorphisms in the FcRIIA and IIIA genes Sylvia MIESCHER*, Martin O SPYCHER, Hanspeter AMSTUTZ, Masja De HAAS, Marion KLEIJER, Ulrich J KALUS, Harmut RADTKE, Alphonse HUBSCH, Irmgard ANDRESEN, Roland M MARTIN, and Johann BICHLER R & D, ZLB Bioplasma AG, BERN, Switzerland; Inselspital, Inst. of Immunology, BERN, Switzerland R & D, ZLB Bioplasma AG, BERN, Switzerland Division CLB, Sanquin, AMSTERDAM, The Netherlands Transfusion Medicine, University Clinics Charite, BERLIN, Germany * Corresponding author; email: sylvia.miescher{at}dkf6.unibe.ch. A single recombinant IgG1 anti-RhD antibody (MonoRho®) was compared with a currently used polyclonal anti-RhD product (Rhophylac®) in a Phase I study for safety, efficacy of Rhesus D positive red blood cell (RBC) clearance and prevention of RhD immunization in RhD negative men challenged with 15ml of RhD positive RBC. Both the polyclonal product and recombinant anti-RhD effectively cleared RhD positive RBC after intravenous and intramuscular injection. The recombinant anti-RhD demonstrated a slower clearance rate compared to the polyclonal anti-RhD. There was no dose response and considerable variation amongst subjects who received the same dose of recombinant anti-RhD. Interestingly, RhD positive RBC clearance rates were strongly associated with FcRIIA, IIIA but not IIIB polymorphisms. Subjects homozygous for FcRIIA-131H or FcRIIIA-158V allotypes showed a faster clearance rate compared to both the heterozygote and the corresponding alternative homozygote allotypes. A similar but less marked trend was seen for the polyclonal anti-RhD. Despite the variation in clearance rates there was no evidence of anti-RhD alloantibodies in any of the subjects at +6 months after the RBC challenge. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Letter in Blood Online: Dose-response relationship and pharmacogenetics of anti-RhD monoclonal antibodies David Ternant, Marc Ohresser, Cécile Thomas, Guillaume Cartron, Hervé Watier, Gilles Paintaud, Alphonse Hubsch, Martin Spycher, Johann Bichler, and Sylvia Miescher Blood 2005 106: 1503-1505. [Full Text] [PDF] This article has been cited by other articles: P. Bruhns, B. Iannascoli, P. England, D. A. Mancardi, N. Fernandez, S. Jorieux, and M. Daeron Specificity and affinity of human Fc{gamma} receptors and their polymorphic variants for human IgG subclasses Blood, April 16, 2009; 113(16): 3716 - 3725. [Abstract] [Full Text] [PDF] M. Shibata-Koyama, S. Iida, A. Okazaki, K. Mori, K. Kitajima-Miyama, S. Saitou, S. Kakita, Y. Kanda, K. Shitara, K. Kato, et al. The N-linked oligosaccharide at Fc{gamma}RIIIa Asn-45: an inhibitory element for high Fc{gamma}RIIIa binding affinity to IgG glycoforms lacking core fucosylation Glycobiology, February 1, 2009; 19(2): 126 - 134. [Abstract] [Full Text] [PDF] M. Kjaersgaard, R. Aslam, M. Kim, E. R. Speck, J. Freedman, D. I. H. Stewart, E. J. Wiersma, and J. W. Semple Epitope specificity and isotype of monoclonal anti-D antibodies dictate their ability to inhibit phagocytosis of opsonized platelets Blood, August 15, 2007; 110(4): 1359 - 1361. [Abstract] [Full Text] [PDF] K. L. Armour, D. R. Parry-Jones, N. Beharry, J. R. Ballinger, R. Mushens, R. K. Williams, C. Beatty, S. Stanworth, P. Lloyd-Evans, M. Scott, et al. Intravascular survival of red cells coated with a mutated human anti-D antibody engineered to lack destructive activity Blood, April 1, 2006; 107(7): 2619 - 2626. [Abstract] [Full Text] [PDF] D. Ternant, M. Ohresser, C. Thomas, G. Cartron, H. Watier, G. Paintaud, A. Hubsch, M. Spycher, J. Bichler, and S. Miescher Dose-response relationship and pharmacogenetics of anti-RhD monoclonal antibodies Blood, August 15, 2005; 106(4): 1503 - 1505. [Full Text] [PDF]

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