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Blood, 15 June 2004, Vol. 103, No. 12, pp. 4588-4593. Prepublished online as a Blood First Edition Paper on February 26, 2004; DOI 10.1182/blood-2003-11-3959. This Article Full Text (PDF) All Versions of this Article: 2003-11-3959v1 103/12/4588    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Lu, J. Articles by Brown, K. E Search for Related Content PubMed PubMed Citation Articles by Lu, J. Articles by Brown, K. E Social Bookmarking                   What's this? Submitted November 18, 2003 Accepted February 9, 2004 Analysis of T cell repertoire in hepatitis-associated aplastic anemia Jun Lu, Atanu Basu, J J Melenhorst, Neal S Young, and Kevin E Brown* Hematology Branch, National Heart Lung and Blood Institute, Bethesda, MD, USA * Corresponding author; email: brownk{at}nhlbi.nih.gov. Hepatitis-associated aplastic anemia (HAA) is a syndrome of bone marrow failure following an acute attack of seronegative hepatitis. Clinical features and liver histology suggest a central role for an immune-mediated mechanism. To characterize the immune response we investigated the T cell repertoire (T cell receptor (TCR) V chain subfamily) of intra-hepatic lymphocytes in HAA patients by TCR spectratyping. In 6/7 HAA liver samples a broad skewing pattern in the 21 V[beta] subfamilies tested was observed. In total, 62% ± 18% of HAA spectratypes showed a skewed pattern, similar to 68% ± 18% skewed spectratype patterns in 3 of 4 patients with confirmed viral hepatitis. Additionally, the T cell repertoire had similarly low levels of complexity. In the PBLs of a separate group of HAA patients prior to treatment, 60% ± 15% skewed spectratypes were detected, compared with only 18% ± 8% skewed spectratypes in healthy controls. After successful immunosuppressive treatment an apparent reversion to a 'normal' T cell repertoire with a corresponding significant increase in T cell repertoire complexity was observed in the HAA samples. In conclusion, our data suggest an antigen-driven T cell expansion in HAA, and achievement of a normal T cell repertoire during recovery from HAA. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. Kenyon and S. L. Gerson The role of DNA damage repair in aging of adult stem cells Nucleic Acids Res., December 26, 2007; (2007) gkm1064v1. [Abstract] [Full Text] [PDF] Y. Osugi, H. Yagasaki, M. Sako, Y. Kosaka, T. Taga, T. Ito, M. Yamamoto, A. Ohara, T. Sato, J. Mimaya, et al. Antithymocyte globulin and cyclosporine for treatment of 44 children with hepatitis associated aplastic anemia Haematologica, December 1, 2007; 92(12): 1687 - 1690. [Abstract] [Full Text] [PDF] C. Fozza, E. Nadal, M. Longinotti, and F. Dazzi T-cell receptor repertoire usage after allografting differs between CD4+CD25+ regulatory T cells and their CD4+CD25 counterpart Haematologica, February 1, 2007; 92(2): 206 - 214. [Abstract] [Full Text] [PDF] N. S. Young, R. T. Calado, and P. Scheinberg Current concepts in the pathophysiology and treatment of aplastic anemia Blood, October 15, 2006; 108(8): 2509 - 2519. [Abstract] [Full Text] [PDF] N. S. Young Pathophysiologic Mechanisms in Acquired Aplastic Anemia Hematology, January 1, 2006; 2006(1): 72 - 77. [Abstract] [Full Text] [PDF]

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