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Blood, 1 November 2004, Vol. 104, No. 9, pp. 2981-2987. Prepublished online as a Blood First Edition Paper on July 15, 2004; DOI 10.1182/blood-2003-11-3961. This Article Full Text (PDF) Supplemental Table All Versions of this Article: 2003-11-3961v1 104/9/2981    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kolar, G. R Articles by Capra, J D Search for Related Content PubMed PubMed Citation Articles by Kolar, G. R Articles by Capra, J D Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted November 19, 2003 Accepted June 21, 2004 Human fetal, cord blood, and adult lymphocyte progenitors have similar potential for generating B cells with a diverse immunoglobulin repertoire Grant R Kolar, Takafumi Yokota, Maria Isabel D Rossi, Swapan K Nath, and J D Capra* Program in Molecular Immunogenetics, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA; Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA Program in Immunobiology and Cancer, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA Program in Arthritis and Immunology, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA * Corresponding author; email: donald-capra{at}omrf.ouhsc.edu. Several characteristics of the immunoglobulin repertoire in fetuses and adults set them apart from each other. Functionally, this translates into differences in the affinity and effectiveness of the humoral immune response between adults and the very young. At least two possibilities could explain these differences: (1) fetal and adult lymphocyte progenitors differ significantly in the recombination potential of the immunoglobulin locus, and (2) factors extrinsic to the immunoglobulin locus are more influential to the character of the repertoire. To address this we used NOD/SCID/2m-/- mice reconstituted with human B cell progenitors to compare the immunoglobulin repertoire potential of human fetal and adult sources. We found nearly identical VH and JH gene segment utilization and modest differences in HCDR3 characteristics. We conclude that the repertoire potential was remarkably similar regardless of the age of the individual from which progenitors are derived. Aging related differences in the immunoglobulin repertoire and variance of B cell responses to immunization appear to arise from selection rather than from changes in recombination of the immunoglobulin locus itself. From the standpoint of the Ig repertoire, an immune system reconstituted from fetal or neonatal stem cells would likely be as diverse as one generated from adult bone marrow. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: G. R. Kolar, D. Mehta, R. Pelayo, and J. D. Capra A novel human B cell subpopulation representing the initial germinal center population to express AID Blood, March 15, 2007; 109(6): 2545 - 2552. [Abstract] [Full Text] [PDF] M. Zemlin, G. Hoersch, C. Zemlin, A. Pohl-Schickinger, M. Hummel, C. Berek, R. F. Maier, and K. Bauer The Postnatal Maturation of the Immunoglobulin Heavy Chain IgG Repertoire in Human Preterm Neonates Is Slower than in Term Neonates J. Immunol., January 15, 2007; 178(2): 1180 - 1188. [Abstract] [Full Text] [PDF]

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