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Blood, 15 August 2004, Vol. 104, No. 4, pp. 1058-1065. Prepublished online as a Blood First Edition Paper on May 6, 2004; DOI 10.1182/blood-2003-11-3979. This Article Full Text (PDF) All Versions of this Article: 2003-11-3979v1 104/4/1058    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Graham, G. J Articles by Gibbins, J. M Search for Related Content PubMed PubMed Citation Articles by Graham, G. J Articles by Gibbins, J. M Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted November 19, 2003 Accepted April 26, 2004 Tachykinins regulate the function of platelets Gwenda J Graham, Joanne M Stevens, Nigel M Page, Andrew D Grant, Susan D Brain, Philip J Lowry, and Jonathan M Gibbins* School of Animal & Microbial Sciences, The University of Reading, Reading, Berkshire, United Kingdom Centre for Cardiovascular Biology and Medicine, King's College, London, United Kingdom * Corresponding author; email: j.m.gibbins{at}reading.ac.uk. Evidence has been mounting for peripheral functions for tachykinins, a family of neuropeptides including substance P (SP), neukokinin A and neurokinin B which are recognised for their roles in the central and peripheral nervous system. The recent discovery of four new members of this family, the endokinins (EKA, B, C and D), which are distributed peripherally, adds support to the notion that tachykinins have physiological/endocrine roles in the periphery. In the present study we report a fundamental new function for tachykinins in the regulation of platelet function. We show that SP stimulates platelet aggregation, and underlying this is the intracellular mobilisation of calcium and degranulation. We demonstrate the presence of the tachykinin receptors NK1 and NK3 in platelets and present evidence for the involvement of NK1 in SP mediated platelet aggregation. Platelets were found to contain SP-like immunoreactivity that is secreted upon activation implicating SP-like substances in the autocrine/paracrine regulation of these cells. Indeed, NK1-blocking antibodies inhibited aggregation in response to other agonists. Of particular note is the observation that EKA/B cross react in the SP immunoassay and are also able to stimulate platelet activation. Together our data implicate tachykinins, specifically SP and EKA/B in the regulation of platelet function. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Platelets feel your pain Robert Flaumenhaft Blood 2004 104: 913. [Full Text] [PDF] This article has been cited by other articles: H. Sun, A. Swaim, J. E. Herrera, D. Becker, L. Becker, K. Srivastava, L. E. Thompson, M. R. Shero, A. Perez-Tamayo, B. Suktitipat, et al. Platelet Kainate Receptor Signaling Promotes Thrombosis by Stimulating Cyclooxygenase Activation Circ. Res., September 11, 2009; 105(6): 595 - 603. [Abstract] [Full Text] [PDF] S. W. S. Sio, M. K. Puthia, J. Lu, S. Moochhala, and M. Bhatia The Neuropeptide Substance P Is a Critical Mediator of Burn-Induced Acute Lung Injury J. Immunol., June 15, 2008; 180(12): 8333 - 8341. [Abstract] [Full Text] [PDF] S. Jones, K. L. Tucker, T. Sage, W. J. Kaiser, N. E. Barrett, P. J. Lowry, A. Zimmer, S. P. Hunt, M. Emerson, and J. M. Gibbins Peripheral tachykinins and the neurokinin receptor NK1 are required for platelet thrombus formation Blood, January 15, 2008; 111(2): 605 - 612. [Abstract] [Full Text] [PDF] I. Hers Insulin-like growth factor-1 potentiates platelet activation via the IRS/PI3K{alpha} pathway Blood, December 15, 2007; 110(13): 4243 - 4252. [Abstract] [Full Text] [PDF]

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