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Blood, 15 February 2005, Vol. 105, No. 4, pp. 1448-1455. Prepublished online as a Blood First Edition Paper on October 26, 2004; DOI 10.1182/blood-2003-11-4068. This Article Full Text (PDF) All Versions of this Article: 2003-11-4068v1 105/4/1448    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Garcon, L. Articles by Dumenil, D. Search for Related Content PubMed PubMed Citation Articles by Garcon, L. Articles by Dumenil, D. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted December 1, 2003 Accepted October 5, 2004 Gfi-1B plays a critical role in terminal differentiation of normal and transformed erythroid progenitor cells Loic Garcon, Catherine Lacout, Fedor Svinartchouk, Jean-Pierre Le Couedic, Jean-Luc Villeval, William Vainchenker, and Dominique Dumenil* INSERM U362, Institut Gustave Roussy, Villejuif, France * Corresponding author; email: dumenil{at}cochin.inserm.fr. Gfi-1B is a transcription factor with a highly conserved transcriptional repressor SNAG domain and six zinc-finger domains at the N and C-terminus, respectively. Disruption of the Gfi-1B gene is lethal in the embryo with failure to produce definitive enucleated erythrocytes. In this study, we analyzed the role of Gfi-1B in human erythropoiesis. We observed an increase of Gfi-1B expression during erythroid maturation of human primary progenitors cells. We studied the consequences of variations in Gfi-1B expression in two transformed cell lines (K562 and UT7 cells), as well as in primary CD36+/GPA- progenitors. A knock-down of Gfi-1B delayed the terminal differentiation of K562 and primary cells. Forced expression of Gfi-1B in UT7 and K562 cells led to an arrest of proliferation and an induction of erythroid differentiation. Enforced expression of Gfi-1B in primary cells at the CFU-E stage led to a partial GPA induction after EPO withdrawal but failed to protect cells from apoptosis. Deletion of the SNAG repressor domain abolished Gfi-1B-induced erythroid maturation, strongly suggesting that Gfi-1B acts in the late stage of erythroid differentiation as a transcriptional repressor. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. Fang, M. Menon, D. Zhang, B. Torbett, L. Oxburgh, M. Tschan, E. Houde, and D. M. Wojchowski Attenuation of EPO-dependent erythroblast formation by death-associated protein kinase-2 Blood, August 1, 2008; 112(3): 886 - 890. [Abstract] [Full Text] [PDF] Y.-Y. Kuo and Z.-F. Chang GATA-1 and Gfi-1B Interplay To Regulate Bcl-xL Transcription Mol. Cell. Biol., June 15, 2007; 27(12): 4261 - 4272. [Abstract] [Full Text] [PDF] L. Vassen, T. Okayama, and T. Moroy Gfi1b:green fluorescent protein knock-in mice reveal a dynamic expression pattern of Gfi1b during hematopoiesis that is largely complementary to Gfi1 Blood, March 15, 2007; 109(6): 2356 - 2364. [Abstract] [Full Text] [PDF] J. A. F. Marteijn, L. T. van der Meer, L. Van Emst, T. de Witte, J. H. Jansen, and B. A. van der Reijden Diminished proteasomal degradation results in accumulation of Gfi1 protein in monocytes Blood, January 1, 2007; 109(1): 100 - 108. [Abstract] [Full Text] [PDF] L. Garcon, C. Rivat, C. James, C. Lacout, V. Camara-Clayette, V. Ugo, Y. Lecluse, A. Bennaceur-Griscelli, and W. Vainchenker Constitutive activation of STAT5 and Bcl-xL overexpression can induce endogenous erythroid colony formation in human primary cells Blood, September 1, 2006; 108(5): 1551 - 1554. [Abstract] [Full Text] [PDF] A. H. Schuh, A. J. Tipping, A. J. Clark, I. Hamlett, B. Guyot, F. J. Iborra, P. Rodriguez, J. Strouboulis, T. Enver, P. Vyas, et al. ETO-2 Associates with SCL in Erythroid Cells and Megakaryocytes and Provides Repressor Functions in Erythropoiesis Mol. Cell. Biol., December 1, 2005; 25(23): 10235 - 10250. [Abstract] [Full Text] [PDF] D.-Y. Huang, Y.-Y. Kuo, and Z.-F. Chang GATA-1 mediates auto-regulation of Gfi-1B transcription in K562 cells Nucleic Acids Res., September 21, 2005; 33(16): 5331 - 5342. [Abstract] [Full Text] [PDF]

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