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Blood, 1 October 2004, Vol. 104, No. 7, pp. 2020-2026. Prepublished online as a Blood First Edition Paper on May 25, 2004; DOI 10.1182/blood-2003-12-4157. This Article Full Text (PDF) All Versions of this Article: 2003-12-4157v1 104/7/2020    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Katayama, Y. Articles by Frenette, P. S Search for Related Content PubMed PubMed Citation Articles by Katayama, Y. Articles by Frenette, P. S Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted December 3, 2003 Accepted May 7, 2004 Integrin 47 and its counterreceptor MAdCAM-1 contribute to hematopoietic progenitor recruitment into bone marrow following transplantation Yoshio Katayama, Andres Hidalgo, Anna J Peired, and Paul S Frenette* Medicine, Mount Sinai School of Medicine, New York, NY, USA; Center for Immunobiology, Mount Sinai School of Medicine, New York, NY, USA * Corresponding author; email: paul.frenette{at}mssm.edu. Previous studies have shown that 41 (VLA-4) and VCAM-1 play a major role in hematopoietic progenitor cell (HPC) homing to bone marrow (BM). However, the antibody used to block VLA-4 function in the mouse (clone PS/2) is not specific to VLA-4 but inhibits both 41 and 47 integrins. Here, we have evaluated the contribution of 47 in HPC homing to BM. LineagenegSca-1posc-kitpos cells from adult mouse BM and the FDCP-mix progenitor cell line express similar levels of 47 by flow cytometry. The 47 complex was functional since the chemokine CXCL12 enhanced the adhesion of FDCP-mix to immobilized MAdCAM-1-Fc, and this was completely abrogated by anti-47 (DATK32) or anti-4 integrins (PS/2). BM intravital microscopy revealed that 47 plays a predominant role in initial tethering and rolling but not in firm adhesion of FDCP-mix cells. Using homing assays, we demonstrate that 47 on HPCs contributes to about half of all 4 integrin-mediated homing activity. MAdCAM-1 is likely expressed since its inhibition significantly reduced HPC homing. Although there may be other 47 integrin ligands involved (e.g. fibronectin and VCAM-1), these data thus suggest that 47 and its counterreceptor MAdCAM-1 represent a novel adhesion pathway that significantly contributes to HPC homing to BM. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: R. Gribi, L. Hook, J. Ure, and A. Medvinsky The differentiation program of embryonic definitive hematopoietic stem cells is largely {alpha}4 integrin independent Blood, July 15, 2006; 108(2): 501 - 509. [Abstract] [Full Text] [PDF] H. Qian, K. Tryggvason, S. E. Jacobsen, and M. Ekblom Contribution of {alpha}6 integrins to hematopoietic stem and progenitor cell homing to bone marrow and collaboration with {alpha}4 integrins Blood, May 1, 2006; 107(9): 3503 - 3510. [Abstract] [Full Text] [PDF] H. Takizawa, C. Kubo-Akashi, I. Nobuhisa, S.-M. Kwon, M. Iseki, T. Taga, K. Takatsu, and S. Takaki Enhanced engraftment of hematopoietic stem/progenitor cells by the transient inhibition of an adaptor protein, Lnk Blood, April 1, 2006; 107(7): 2968 - 2975. [Abstract] [Full Text] [PDF] E. Waldman, S. X. Lu, V. M. Hubbard, A. A. Kochman, J. M. Eng, T. H. Terwey, S. J. Muriglan, T. D. Kim, G. Heller, G. F. Murphy, et al. Absence of beta7 integrin results in less graft-versus-host disease because of decreased homing of alloreactive T cells to intestine Blood, February 15, 2006; 107(4): 1703 - 1711. [Abstract] [Full Text] [PDF] G. Qin, M. Ii, M. Silver, A. Wecker, E. Bord, H. Ma, M. Gavin, D. A. Goukassian, Y.-s. Yoon, T. Papayannopoulou, et al. Functional disruption of {alpha}4 integrin mobilizes bone marrow-derived endothelial progenitors and augments ischemic neovascularization J. Exp. Med., January 23, 2006; 203(1): 153 - 163. [Abstract] [Full Text] [PDF] S. K. Nilsson, H. M. Johnston, G. A. Whitty, B. Williams, R. J. Webb, D. T. Denhardt, I. Bertoncello, L. J. Bendall, P. J. Simmons, and D. N. Haylock Osteopontin, a key component of the hematopoietic stem cell niche and regulator of primitive hematopoietic progenitor cells Blood, August 15, 2005; 106(4): 1232 - 1239. [Abstract] [Full Text] [PDF]

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