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Blood, 15 June 2004, Vol. 103, No. 12, pp. 4581-4587. Prepublished online as a Blood First Edition Paper on February 5, 2004; DOI 10.1182/blood-2003-12-4172. This Article Full Text (PDF) All Versions of this Article: 2003-12-4172v1 103/12/4581    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Bahbouhi, B. Articles by Al-Harthi, L. Search for Related Content PubMed PubMed Citation Articles by Bahbouhi, B. Articles by Al-Harthi, L. Social Bookmarking                   What's this? Submitted December 8, 2003 Accepted January 29, 2004 Dynamics of cytokine expression within HIV productively infected primary CD4+ T cells Bouchaib Bahbouhi, Alan Landay, and Lena Al-Harthi* Immunology/Microbiology, Rush University Medical Center, Chicago, IL, USA * Corresponding author; email: lalharth{at}rush.edu. Using intracellular p24 staining to discriminate between bystander and HIV productively infected cells, we evaluated the properties of HIV productively infected cells in terms of cytokine expression, activation status, apoptosis, and cell proliferation. We demonstrate that HIV productively infected primary CD4+ T cells express 12-47-fold higher type 1 cytokines than bystander or mock infected cells. The frequency of HIV productive replication occurred predominately within Th1, followed by TH0, then by TH2 cells. These productively infected cells expressed elevated levels of CD95, CD25, CXCR4, and CCR5. While productively infected cells were only 1.8-fold higher in apoptosis frequency, they up-regulated the anti-apoptotic protein Bcl-2 by 10-fold. Up-regulation of IL-2 and Bcl-2 were dependant on phosphatidylinositol-3-kinase signal transduction, given that it was down-regulated by Worthmanin treatment. Additionally, 60% of productively infected cells entered the cell cycle, as evaluated by Ki67 staining, but none divided, as was evaluated by CFSE staining. Evaluation of cell cycle progression by co-staining for DNA and RNA indicated that the cells were arrested in G2/M. Collectively, this data indicate that HIV replication occurs predominately in Th1 cells, is associated with immune activation and up-regulation of Bcl-2, conferring a considerable degree of protection against apoptosis within the productively infected subpopulation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. Choi, J. Walker, K. Talbert-Slagle, P. Wright, J. S. Pober, and L. Alexander Endothelial Cells Promote Human Immunodeficiency Virus Replication in Nondividing Memory T Cells via Nef-, Vpr-, and T-Cell Receptor-Dependent Activation of NFAT J. Virol., September 1, 2005; 79(17): 11194 - 11204. [Abstract] [Full Text] [PDF]

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