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Blood, 1 January 2005, Vol. 105, No. 1, pp. 95-102. Prepublished online as a Blood First Edition Paper on September 14, 2004; DOI 10.1182/blood-2003-12-4345. This Article Full Text (PDF) All Versions of this Article: 2003-12-4345v1 105/1/95    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Masuya, M. Articles by Ogawa, M. Search for Related Content PubMed PubMed Citation Articles by Masuya, M. Articles by Ogawa, M. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted December 22, 2003 Accepted August 1, 2004 Dysregulation of granulocyte, erythrocyte and NK cell lineages in Fli-1 gene targeted mice Masahiro Masuya, Omar Moussa, Takanori Abe, Takao Deguchi, Tsukasa Higuchi, Yasuhiro Ebihara, Demetri D Spyropoulos, Dennis K Watson, and Makio Ogawa* Department of Veterans Affairs Medical Center, Charleston, SC, USA; Department of Medicine, Medical University of South Carolina, Charleston, SC, USA Department of Pathology, Medical University of South Carolina, Charleston, SC, USA; Hollings Cancer Center, Medical University of South Carolina, Charleston, SC, USA Hollings Cancer Center, Medical University of South Carolina, Charleston, SC, USA Department of Pathology, Medical University of South Carolina, Charleston, SC, USA; Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC, USA; Hollings Cancer Center, Medical University of South Carolina, Charleston, SC, USA * Corresponding author; email: ogawam{at}musc.edu. Targeted disruption of Fli-1 proto-oncogene results in severe dysmegakaryopoiesis and embryonic lethality. We used morula-stage aggregation as a strategy to clarify further the hematopoietic defects of the Fli-1 gene-targeted mice. Analyses of lineage expression of Fli-1+/- and Fli-1-/- cells in the peripheral blood and bone marrow of chimeric mice consistently demonstrated reduced numbers of neutrophilic granulocytes and monocytes and increased numbers of NK cells. Transplantation studies using sorted Fli-1 mutant cells produced similar findings. Clonal culture studies of bone marrow cells revealed increased numbers of granulocytic and early erythroid progenitors in the Fli-1+/- cells. The sorted Fli-1-/- bone marrow cells revealed specific down-regulation of C/EBP and C/EBP, and the receptors for G-CSF and GM-CSF, consistent with their critical roles in granulopoiesis. Collectively, these observations suggest previously unknown physiological roles for Fli-1 in granulocytic, erythroid and NK cell proliferation and differentiation. Production of chimeras by morula-stage embryo aggregation is an effective way to unravel cell-autonomous hematopoietic defects in gene-targeted mice. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: A Fli in the ointment Ramesh A. Shivdasani Blood 2005 105: 9-10. [Full Text] [PDF] This article has been cited by other articles: Y. Asano and M. Trojanowska Phosphorylation of Fli1 at Threonine 312 by Protein Kinase C {delta} Promotes Its Interaction with p300/CREB-Binding Protein-Associated Factor and Subsequent Acetylation in Response to Transforming Growth Factor {beta} Mol. Cell. Biol., April 1, 2009; 29(7): 1882 - 1894. [Abstract] [Full Text] [PDF] Y. Asano, M. Markiewicz, M. Kubo, G. Szalai, D. K. Watson, and M. Trojanowska Transcription Factor Fli1 Regulates Collagen Fibrillogenesis in Mouse Skin Mol. Cell. Biol., January 15, 2009; 29(2): 425 - 434. [Abstract] [Full Text] [PDF] F. Bouilloux, G. Juban, N. Cohet, D. Buet, B. Guyot, W. Vainchenker, F. Louache, and F. Morle EKLF restricts megakaryocytic differentiation at the benefit of erythrocytic differentiation Blood, August 1, 2008; 112(3): 576 - 584. [Abstract] [Full Text] [PDF] Y. Asano, J. Czuwara, and M. Trojanowska Transforming Growth Factor- Regulates DNA Binding Activity of Transcription Factor Fli1 by p300/CREB-binding Protein-associated Factor-dependent Acetylation J. Biol. Chem., November 30, 2007; 282(48): 34672 - 34683. [Abstract] [Full Text] [PDF] S. S. Nakerakanti, B. Kapanadze, M. Yamasaki, M. Markiewicz, and M. Trojanowska Fli1 and Ets1 Have Distinct Roles in Connective Tissue Growth Factor/CCN2 Gene Regulation and Induction of the Profibrotic Gene Program J. Biol. Chem., September 1, 2006; 281(35): 25259 - 25269. [Abstract] [Full Text] [PDF] E. van den Akker, S. Ano, H.-M. Shih, L.-C. Wang, M. Pironin, J. J. Palvimo, N. Kotaja, O. Kirsh, A. Dejean, and J. Ghysdael FLI-1 Functionally Interacts with PIASx{alpha}, a Member of the PIAS E3 SUMO Ligase Family J. Biol. Chem., November 11, 2005; 280(45): 38035 - 38046. [Abstract] [Full Text] [PDF]

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