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Blood, 1 March 2005, Vol. 105, No. 5, pp. 2235-2238. Prepublished online as a Blood First Edition Paper on August 3, 2004; DOI 10.1182/blood-2003-12-4399. This Article Full Text (PDF) All Versions of this Article: 2003-12-4399v1 105/5/2235    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Craiu, A. Articles by Scadden, D. T Search for Related Content PubMed PubMed Citation Articles by Craiu, A. Articles by Scadden, D. T Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted January 2, 2004 Accepted July 14, 2004 Flowing cells through pulsed electric fields efficiently purges stem cell preparations of contaminating myeloma cells while preserving stem cell function Abie Craiu, Yoriko Saito, Ana Limon, Henry M Eppich, Douglas P Olson, Neil Rodrigues, Gergor B Adams, David Dombkowski, Paul Richardson, Robert Schlossman, Peter S Choi, Jonathan Grogins, Paula G O'Connor, Kenneth Cohen, Eyal C Attar, Jay Freshman, Rebecca Rich, Joseph A Mangano, John G Gribben, Kenneth C Anderson, and David T Scadden* Science Research Laboratory, Inc., Somerville, MA, USA Center for Regenerative Medicine and Technology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA Jerome Lipper Myeloma Center, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA, USA Science Research Laboratory, Inc., Somerville, MA, USA; Center for Regenerative Medicine and Technology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA * Corresponding author; email: scadden.david{at}mgh.harvard.edu. Autologous stem cell transplantation, in the setting of hematologic malignancies such as lymphoma, improves disease free survival if the graft has undergone tumor purging. Here we show that flowing hematopoietic cells through pulsed electric fields (PEF) effectively purges myeloma cells without sacrificing functional stem cells. Electric fields can induce irreversible cell membrane pores in direct relation to cell diameter, an effect we exploit in a flowing system appropriate for clinical scale. Multiple myeloma (MM) cell lines admixed with human BM or PB cells were passed through PEF at 1.35 to 1.4 kV/cm, resulting in 3 to 4-log tumor cell depletion by flow cytometry and 4.5 to 6-log depletion by tumor regrowth cultures. Samples from patients with MM gave similar results by cytometry. Stem cell engraftment into NOD/SCID/B2m-/- mice was unperturbed by PEF. Flowing cells through PEF is a promising technology for rapid tumor cell purging of clinical progenitor cell preparations. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

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