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Blood, 15 June 2004, Vol. 103, No. 12, pp. 4644-4649. Prepublished online as a Blood First Edition Paper on February 26, 2004; DOI 10.1182/blood-2003-12-4412. This Article Full Text (PDF) All Versions of this Article: 2003-12-4412v1 103/12/4644    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ladetto, M. Articles by Tarella, C. Search for Related Content PubMed PubMed Citation Articles by Ladetto, M. Articles by Tarella, C. Social Bookmarking                   What's this? Submitted December 30, 2003 Accepted February 9, 2004 Telomere length correlates with histopathogenesis according to the germinal center in mature B-cell lymphoproliferative disorders Marco Ladetto*, Mara Compagno, Irene Ricca, Marco Pagano, Alberto Rocci, Monica Astolfi, Daniela Drandi, Paola Francia di Celle, Maria Dell Aquila, Barbara Mantoan, Sonia Vallet, Gloria Pagliano, Federica De Marco, Roberto Francese, Loredana Santo, Alessandra Cuttica, Carlo Marinone, Mario Boccadoro, and Corrado Tarella Divisione di Ematologia, Dipartimento di Medicina ed Oncologia Sperimentale, Universita di Torino, Azienda Ospedaliera S. Giovanni Battista, Torino, Italy Divisione di Anatomia Patologica, Dipartimento di Scienze Biomediche ed Oncologia Umana, Universita di Torino, Azienda Ospedaliera S. Giovanni Battista, Torino, Italy Divisione di Medicina Generale VII, Azienda Ospedaliera S. Giovanni Battista, Torino, Italy * Corresponding author; email: marco.ladetto{at}unito.it. In this study we investigated telomere restriction fragment (TRF) length in a panel of mature B cell lymphoproliferative disorders (MBCLD) and correlated this parameter with histology and histopathogenesis in relation to the germinal center (GC). We assessed 123 MBCLD samples containing 80% tumor cells. TRF length was evaluated by Southern blot using a chemiluminescence-based assay. GC-status was assessed through screening for stable and ongoing somatic mutations within the immunoglobulin heavy chain genes. Median TRF length was 6170 bp (range 1896-11200 bp) and did not correlate with patient age and sex. TRF length was greater in diffuse large cell, Burkitt's and follicular lymphoma (median: 7789 bp, 9471 bp, 7383 bp, respectively) than in mantle cell lymphoma and chronic lymphocytic leukemia (median: 3582 bp and 4346 bp, respectively). GC-derived MBCLD have the longest telomeres, while those arising from GC-inexperienced cells have the shortest (p<10-9). We conclude that: 1) TRF length in MBCLD is highly heterogeneous; 2) GC-derived tumors have long telomeres suggesting that minimal telomere erosion occurs during GC-derived lymphomagenesis; 3) the low TRF length of GC-inexperienced MBCLD indicates that these neoplasms are good candidates for treatment with telomerase inhibitors, a class of molecules currently the subject of extensive pre-clinical evaluation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. H. Ghaffari, N. Shayan-Asl, A. H. Jamialahmadi, K. Alimoghaddam, and A. Ghavamzadeh Telomerase activity and telomere length in patients with acute promyelocytic leukemia: indicative of proliferative activity, disease progression, and overall survival Ann. Onc., November 1, 2008; 19(11): 1927 - 1934. [Abstract] [Full Text] [PDF] G. Roos, A. Krober, P. Grabowski, D. Kienle, A. Buhler, H. Dohner, R. Rosenquist, and S. Stilgenbauer Short telomeres are associated with genetic complexity, high-risk genomic aberrations, and short survival in chronic lymphocytic leukemia Blood, February 15, 2008; 111(4): 2246 - 2252. [Abstract] [Full Text] [PDF] D. Poncet, A. Belleville, C. t'Kint de Roodenbeke, A. Roborel de Climens, E. Ben Simon, H. Merle-Beral, E. Callet-Bauchu, G. Salles, L. Sabatier, J. Delic, et al. Changes in the expression of telomere maintenance genes suggest global telomere dysfunction in B-chronic lymphocytic leukemia Blood, February 15, 2008; 111(4): 2388 - 2391. [Abstract] [Full Text] [PDF] P. Grabowski, M. Hultdin, K. Karlsson, G. Tobin, A. Aleskog, U. Thunberg, A. Laurell, C. Sundstrom, R. Rosenquist, and G. Roos Telomere length as a prognostic parameter in chronic lymphocytic leukemia with special reference to VH gene mutation status Blood, June 15, 2005; 105(12): 4807 - 4812. [Abstract] [Full Text] [PDF]

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