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Blood, 1 July 2004, Vol. 104, No. 1, pp. 89-91. Prepublished online as a Blood First Edition Paper on March 11, 2004; DOI 10.1182/blood-2004-01-0108. This Article Full Text (PDF) Supplemental Table All Versions of this Article: 2004-01-0108v1 104/1/89    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Guyot, B. Articles by Vyas, P. Search for Related Content PubMed PubMed Citation Articles by Guyot, B. Articles by Vyas, P. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted January 12, 2004 Accepted February 29, 2004 Deletion of the major GATA1 enhancer HS 1 does not affect eosinophil GATA1 expression and eosinophil differentiation Boris Guyot, Veronica Valverde-Garduno, Catherine Porcher, and Paresh Vyas* Weatherall Institute of Molecular Medicine, University of Oxford and Oxford Radcliffe Hospitals, Oxford, United Kingdom MRC Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, United Kingdom * Corresponding author; email: paresh.vyas{at}imm.ox.ac.uk. Expression of the myeloid transcription factor GATA1 is required at early stages of eosinophil differentiation. Defining mechanisms regulating eosinophil GATA1 expression will be important to understand development of this lineage. However, the cis-elements required for eosinophil GATA1 expression are not fully characterised. In particular, the role of HS 1, a major GATA1 enhancer, in eosinophil GATA1 expression is unclear. Here, we show that mouse HS 1 deletion does not perturb eosinophil GATA1 mRNA expression and eosinophil differentiation. Primary eosinophil chromatin encompassing HS 1 is weakly enriched for acetylated histones H3/H4, and HS 1 deletion does not alter GATA1 locus histone acetylation. Moreover, in vivo, GATA1 and C/EBP bind a cis-element in a GATA1 intron and the GATA1 promoter, but neither binds HS 1. Thus, HS 1 is not required for eosinophil GATA1 expression. Instead, this study suggests a previously unsuspected role for the GATA1 intron element for this function. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. Milanovic, G. Terszowski, D. Struck, O. Liesenfeld, and D. Carstanjen IFN Consensus Sequence Binding Protein (Icsbp) Is Critical for Eosinophil Development J. Immunol., October 1, 2008; 181(7): 5045 - 5053. [Abstract] [Full Text] [PDF] I. Hamlett, J. Draper, J. Strouboulis, F. Iborra, C. Porcher, and P. Vyas Characterization of megakaryocyte GATA1-interacting proteins: the corepressor ETO2 and GATA1 interact to regulate terminal megakaryocyte maturation Blood, October 1, 2008; 112(7): 2738 - 2749. [Abstract] [Full Text] [PDF] M. Kobayashi and M. Yamamoto Regulation of GATA1 Gene Expression J. Biochem., July 1, 2007; 142(1): 1 - 10. [Abstract] [Full Text] [PDF] N. Meier, S. Krpic, P. Rodriguez, J. Strouboulis, M. Monti, J. Krijgsveld, M. Gering, R. Patient, A. Hostert, and F. Grosveld Novel binding partners of Ldb1 are required for haematopoietic development Development, December 15, 2006; 133(24): 4913 - 4923. [Abstract] [Full Text] [PDF] F. Bose, C. Fugazza, M. Casalgrandi, A. Capelli, J. M. Cunningham, Q. Zhao, S. M. Jane, S. Ottolenghi, and A. Ronchi Functional Interaction of CP2 with GATA-1 in the Regulation of Erythroid Promoters Mol. Cell. Biol., May 15, 2006; 26(10): 3942 - 3954. [Abstract] [Full Text] [PDF] B. Guyot, K. Murai, Y. Fujiwara, V. Valverde-Garduno, M. Hammett, S. Wells, N. Dear, S. H. Orkin, C. Porcher, and P. Vyas Characterization of a Megakaryocyte-specific Enhancer of the Key Hemopoietic Transcription Factor GATA1 J. Biol. Chem., May 12, 2006; 281(19): 13733 - 13742. [Abstract] [Full Text] [PDF] C. Kuhl, A. Atzberger, F. Iborra, B. Nieswandt, C. Porcher, and P. Vyas GATA1-Mediated Megakaryocyte Differentiation and Growth Control Can Be Uncoupled and Mapped to Different Domains in GATA1 Mol. Cell. Biol., October 1, 2005; 25(19): 8592 - 8606. [Abstract] [Full Text] [PDF] R. Ferreira, K. Ohneda, M. Yamamoto, and S. Philipsen GATA1 Function, a Paradigm for Transcription Factors in Hematopoiesis Mol. Cell. Biol., February 15, 2005; 25(4): 1215 - 1227. [Full Text] [PDF]

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