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Blood, 1 October 2004, Vol. 104, No. 7, pp. 2116-2123. Prepublished online as a Blood First Edition Paper on June 3, 2004; DOI 10.1182/blood-2004-01-0150. This Article Full Text (PDF) All Versions of this Article: 2004-01-0150v1 104/7/2116    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Dhanji, S. Articles by Teh, H.-S. Search for Related Content PubMed PubMed Citation Articles by Dhanji, S. Articles by Teh, H.-S. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted January 14, 2004 Accepted May 13, 2004 Self-Reactive Memory-Phenotype CD8 T Cells Exhibit Both MHC-Restricted and Non-MHC Restricted Cytotoxicity: A Role for the T Cell Receptor and Natural Killer Cell Receptors Salim Dhanji, Soo-Jeet Teh, Darryl Oble, John J Priatel, and Hung-Sia Teh* Microbiology and Immunology, University of British Columbia, Vancouver, BC, Canada * Corresponding author; email: teh{at}interchange.ubc.ca. We have recently shown that IL-2-activated CD8+CD44hi cells from normal mice express both adaptive and innate immune system receptors and specifically kill syngeneic tumor cells, particularly those that express NKG2D ligands. Here we show that CD8+ T cells from antigen-expressing H-Y TCR transgenic mice also exhibit characteristics of both T cells and NK cells. Interaction with cognate self-antigen was required for the optimal expansion of these cells in peripheral lymphoid tissues. Although these cells possess a higher activation threshold relative to naive T cells, they can be activated by cytokine alone in vitro. They also undergo bystander proliferation in response to a bacterial infection in vivo. Interestingly, upon activation, the cells express the NKG2D receptor as well as the DAP12 adaptor protein. We provide evidence that NKG2D can act additively with the TCR in the killing of target cells and it can also function as a directly activating receptor in non-MHC restricted killing of target cells. These properties of CD8+ T cells from H-Y TCR transgenic mice are remarkably similar to CD8+CD44hi cells that are found in normal mice. The H-Y TCR transgenic mice provide a well-defined system for characterizing the developmental biology and function of these cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: E. Y. Kim, S.-J. Teh, J. Yang, M. T. Chow, and H.-S. Teh TNFR2-Deficient Memory CD8 T Cells Provide Superior Protection against Tumor Cell Growth J. Immunol., November 15, 2009; 183(10): 6051 - 6057. [Abstract] [Full Text] [PDF] W. Cao, X. Xi, Z. Wang, L. Dong, Z. Hao, L. Cui, C. Ma, and W. He Four novel ULBP splice variants are ligands for human NKG2D Int. Immunol., August 1, 2008; 20(8): 981 - 991. [Abstract] [Full Text] [PDF] A. Iannello, O. Debbeche, S. Samarani, and A. Ahmad Antiviral NK cell responses in HIV infection: I. NK cell receptor genes as determinants of HIV resistance and progression to AIDS J. Leukoc. Biol., July 1, 2008; 84(1): 1 - 26. [Abstract] [Full Text] [PDF] X. Chen, J. J. Priatel, M. T. Chow, and H.-S. Teh Preferential Development of CD4 and CD8 T Regulatory Cells in RasGRP1-Deficient Mice J. Immunol., May 1, 2008; 180(9): 5973 - 5982. [Abstract] [Full Text] [PDF] W. Cao, X. Xi, Z. Hao, W. Li, Y. Kong, L. Cui, C. Ma, D. Ba, and W. He RAET1E2, a Soluble Isoform of the UL16-binding Protein RAET1E Produced by Tumor Cells, Inhibits NKG2D-mediated NK Cytotoxicity J. Biol. Chem., June 29, 2007; 282(26): 18922 - 18928. [Abstract] [Full Text] [PDF] S. Dubois, T. A. Waldmann, and J. R. Muller ITK and IL-15 support two distinct subsets of CD8+ T cells PNAS, August 8, 2006; 103(32): 12075 - 12080. [Abstract] [Full Text] [PDF] S. Dhanji, M. T. Chow, and H.-S. Teh Self-Antigen Maintains the Innate Antibacterial Function of Self-Specific CD8 T Cells In Vivo J. Immunol., July 1, 2006; 177(1): 138 - 146. [Abstract] [Full Text] [PDF] M. W. L. Teng, M. H. Kershaw, Y. Hayakawa, L. Cerutti, S. M. Jane, P. K. Darcy, and M. J. Smyth T Cells Gene-engineered with DAP12 Mediate Effector Function in an NKG2D-dependent and Major Histocompatibility Complex-independent Manner J. Biol. Chem., November 18, 2005; 280(46): 38235 - 38241. [Abstract] [Full Text] [PDF] J. Su, R. E. Berg, S. Murray, and J. Forman Thymus-Dependent Memory Phenotype CD8 T Cells in Naive B6.H-2Kb-/-Db-/- Animals Mediate an Antigen-Specific Response against Listeria monocytogenes J. Immunol., November 15, 2005; 175(10): 6450 - 6457. [Abstract] [Full Text] [PDF] B. Rincon-Orozco, V. Kunzmann, P. Wrobel, D. Kabelitz, A. Steinle, and T. Herrmann Activation of V{gamma}9V{delta}2 T Cells by NKG2D J. Immunol., August 15, 2005; 175(4): 2144 - 2151. [Abstract] [Full Text] [PDF] L. A. Pitcher, M. A. Mathis, S. Subramanian, J. A. Young, E. K. Wakeland, P. E. Love, and N. S. C. van Oers Selective Expression of the 21-Kilodalton Tyrosine-Phosphorylated Form of TCR {zeta} Promotes the Emergence of T Cells with Autoreactive Potential J. Immunol., May 15, 2005; 174(10): 6071 - 6079. [Abstract] [Full Text] [PDF] L. I. R. Ehrlich, K. Ogasawara, J. A. Hamerman, R. Takaki, A. Zingoni, J. P. Allison, and L. L. Lanier Engagement of NKG2D by Cognate Ligand or Antibody Alone Is Insufficient to Mediate Costimulation of Human and Mouse CD8+ T Cells J. Immunol., February 15, 2005; 174(4): 1922 - 1931. [Abstract] [Full Text] [PDF] S. Dhanji, K. Tse, and H.-S. Teh The Low Affinity Fc Receptor for IgG Functions as an Effective Cytolytic Receptor for Self-Specific CD8 T Cells J. Immunol., February 1, 2005; 174(3): 1253 - 1258. [Abstract] [Full Text] [PDF]

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