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Blood, 1 August 2004, Vol. 104, No. 3, pp. 711-718. Prepublished online as a Blood First Edition Paper on April 15, 2004; DOI 10.1182/blood-2004-01-0254. This Article Full Text (PDF) All Versions of this Article: 2004-01-0254v1 104/3/711    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Hirashima, M. Articles by Rossant, J. Search for Related Content PubMed PubMed Citation Articles by Hirashima, M. Articles by Rossant, J. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted January 22, 2004 Accepted March 23, 2004 Gene trap expression screening to identify endothelial-specific genes Masanori Hirashima, Alan Bernstein, William L Stanford, and Janet Rossant* Samuel Lunenfeld Research Institute, Toronto, Ontario, Canada Samuel Lunenfeld Research Institute, Toronto, Ontario, Canada; Molecular and Medical Genetics, University of Toronto, Toronto, Ontario, Canada Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada * Corresponding author; email: rossant{at}mshri.on.ca. The endothelial cell is a key cellular component for blood vessel formation. Many signaling receptors expressed in endothelial cells play critical roles in vascular development during embryogenesis. However, downstream response genes required for vascular differentiation are still not clearly identified. Here we describe the development of a protocol for gene trap expression screening in embryonic stem (ES) cells for endothelial-specific genes. ES cells were differentiated into endothelial cells on an OP9 feeder cell layer in 96-well plates. In a pilot screen, five gene trapped ES cell lines showed an upregulated expression of the gene trap lacZ reporter out of 864 ES clones screened. One of the trapped genes was Endoglin, an endothelial-specific TGF- type III receptor, and another was ASPP1, a p53-binding protein. In vivo expression analysis of the lacZ reporter confirmed that both genes are specifically expressed in endothelial cells during early mouse embryogenesis. Gene trap expression screening can thus be used to identify endothelial-specific genes and analyze their function in mice. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Trapping endothelial-specific genes Johannes Roth Blood 2004 104: 599. [Full Text] [PDF] This article has been cited by other articles: T. Lee, C. Shah, and E. Y. Xu Gene trap mutagenesis: a functional genomics approach towards reproductive research Mol. Hum. Reprod., November 1, 2007; 13(11): 771 - 779. [Abstract] [Full Text] [PDF] R. C. R. Perlingeiro Endoglin is required for hemangioblast and early hematopoietic development Development, August 15, 2007; 134(16): 3041 - 3048. [Abstract] [Full Text] [PDF] A. T. Quinones-Coello, L. N. Petrella, K. Ayers, A. Melillo, S. Mazzalupo, A. M. Hudson, S. Wang, C. Castiblanco, M. Buszczak, R. A. Hoskins, et al. Exploring Strategies for Protein Trapping in Drosophila Genetics, March 1, 2007; 175(3): 1089 - 1104. [Abstract] [Full Text] [PDF] Y. Liu and A. Deisseroth Tumor vascular targeting therapy with viral vectors Blood, April 15, 2006; 107(8): 3027 - 3033. [Abstract] [Full Text] [PDF] T. Kamata, J. Kang, T.-H. Lee, L. Wojnowski, C. A. Pritchard, and A. D. Leavitt A critical function for B-Raf at multiple stages of myelopoiesis Blood, August 1, 2005; 106(3): 833 - 840. [Abstract] [Full Text] [PDF]

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