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Blood, 1 November 2004, Vol. 104, No. 9, pp. 2832-2839. Prepublished online as a Blood First Edition Paper on July 8, 2004; DOI 10.1182/blood-2004-01-0268. This Article Full Text (PDF) All Versions of this Article: 2004-01-0268v1 104/9/2832    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sloane, D. E Articles by Arm, J. P Search for Related Content PubMed PubMed Citation Articles by Sloane, D. E Articles by Arm, J. P Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted January 23, 2004 Accepted June 21, 2004 Leukocyte Immunoglobulin-like Receptors: novel innate receptors for human basophil activation and inhibition David E Sloane, Nicodemus Tedla, Muyiwa Awoniyi, Donald W MacGlashan, Luis Borges, K F Austen, and Jonathan P Arm* Division of Rheumatology Immunology and Allergy, Brigham and Women's Hospital, Boston, MA, USA; Department of Medicine, Harvard Medical School, Boston, MA, USA; Partners Asthma Center, Brigham and Women's Hospital, Boston, MA, USA Division of Rheumatology Immunology and Allergy, Brigham and Women's Hospital, Boston, MA, USA; Department of Medicine, Harvard Medical School, Boston, MA, USA Division of Rheumatology Immunology and Allergy, Brigham and Women's Hospital, Boston, MA, USA Asthma and Allergy Center, Johns Hopkins Hospital, Baltimore, MD, USA Amgen Inc., Seattle, WA, USA Department of Medicine, Harvard Medical School, Boston, MA, USA; Division of Rheumatology Immunology and Allergy, Brigham and Women's Hospital, Boston, MA, USA; Partners Asthma Center, Brigham and Women's Hospital, Boston, MA, USA * Corresponding author; email: jarm{at}rics.bwh.harvard.edu. Basophils, recruited from the blood to tissues, have been implicated by their presence in diverse allergic disorders including bronchial asthma, allergic rhinitis, and cutaneous contact hypersensitivity. We hypothesized that like other leukocytes involved in inflammatory responses, basophils would express members of the Leukocyte Immunogloblin-like Receptor (LIR) family of immunoregulatory molecules on their cell surface. We identified LIR7, an activating member coupled to the common Fc receptor gamma chain, and LIR3, an inhibitory member containing cytoplasmic immunoreceptor tyrosine-based inhibitory motifs on these cells from human peripheral blood. Cross-linking of LIR7 resulted in the concentration-dependent net release of histamine (29.8±10.8%) and cysteinyl leukotrienes (cysLT) (31.4±8.7 ng/106 basophils) that were maximal at 30 minutes, and of Interleukin (IL) 4 (410.2±61.6 pg/106 basophils) that was maximal at 4 hours and comparable to the response initiated by cross-linking of the high affinity receptor for IgE (FcRI). Co-ligation of LIR3 to LIR7 or to FcRI by means of a second monoclonal antibody significantly inhibited net histamine release, cysLT production, and IL-4 generation. That LIR3 is profoundly counter-regulatory for both adaptive and innate receptors suggests a broad role in containment of the inflammatory response. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D. W. MacGlashan Jr., S. Ishmael, S. M. MacDonald, J. M. Langdon, J. P. Arm, and D. E. Sloane Induced Loss of Syk in Human Basophils by Non-IgE-Dependent Stimuli J. Immunol., March 15, 2008; 180(6): 4208 - 4217. [Abstract] [Full Text] [PDF] N. Tedla, C.-W. Lee, L. Borges, C. L. Geczy, and J. P. Arm Differential expression of leukocyte immunoglobulin-like receptors on cord blood-derived human mast cell progenitors and mature mast cells J. Leukoc. Biol., February 1, 2008; 83(2): 334 - 343. [Abstract] [Full Text] [PDF] O. A. Huynh, T. Hampartzoumian, J. P. Arm, J. Hunt, L. Borges, M. Ahern, M. Smith, C. L. Geczy, H. P. McNeil, and N. Tedla Down-regulation of leucocyte immunoglobulin-like receptor expression in the synovium of rheumatoid arthritis patients after treatment with disease-modifying anti-rheumatic drugs Rheumatology, May 1, 2007; 46(5): 742 - 751. [Abstract] [Full Text] [PDF] A. Masuda, A. Nakamura, T. Maeda, Y. Sakamoto, and T. Takai Cis binding between inhibitory receptors and MHC class I can regulate mast cell activation J. Exp. Med., April 16, 2007; 204(4): 907 - 920. [Abstract] [Full Text] [PDF] M. Shiroishi, M. Kajikawa, K. Kuroki, T. Ose, D. Kohda, and K. Maenaka Crystal Structure of the Human Monocyte-activating Receptor, "Group 2" Leukocyte Ig-like Receptor A5 (LILRA5/LIR9/ILT11) J. Biol. Chem., July 14, 2006; 281(28): 19536 - 19544. [Abstract] [Full Text] [PDF] A. Munitz, I. Bachelet, R. Eliashar, A. Moretta, L. Moretta, and F. Levi-Schaffer The inhibitory receptor IRp60 (CD300a) suppresses the effects of IL-5, GM-CSF, and eotaxin on human peripheral blood eosinophils Blood, March 1, 2006; 107(5): 1996 - 2003. [Abstract] [Full Text] [PDF] Y. Arinobu, H. Iwasaki, M. F. Gurish, S.-i. Mizuno, H. Shigematsu, H. Ozawa, D. G. Tenen, K. F. Austen, and K. Akashi Developmental checkpoints of the basophil/mast cell lineages in adult murine hematopoiesis PNAS, December 13, 2005; 102(50): 18105 - 18110. [Abstract] [Full Text] [PDF]

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