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Blood, 15 October 2004, Vol. 104, No. 8, pp. 2397-2402. Prepublished online as a Blood First Edition Paper on June 24, 2004; DOI 10.1182/blood-2004-01-0324. This Article Full Text (PDF) All Versions of this Article: 2004-01-0324v1 104/8/2397    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Yuan, R. R Articles by Scheinberg, D. A Search for Related Content PubMed PubMed Citation Articles by Yuan, R. R Articles by Scheinberg, D. A Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted January 29, 2004 Accepted May 17, 2004 Targeted deletion of T cell clones using alpha emitting suicide MHC tetramers Rui R Yuan, Phillip Wong, Michael R McDevitt, Ekaterina Doubrovina, Ingrid Leiner, William Bornmann, Richard O'Reilly, Eric G Pamer, and David A Scheinberg* Molecular Pharmacology and Chemistry, Sloan-Kettering Institute and Memorial Sloan-Kettering Cancer Center, New York, NY, USA Medicine, Sloan-Kettering Institute and Memorial Sloan-Kettering Cancer Center, New York, NY, USA Pediatric, Sloan-Kettering Institute and Memorial Sloan-Kettering Cancer Center, New York, NY, USA Molecular Pharmacology and Chemistry, Sloan-Kettering Institute and Memorial Sloan-Kettering Cancer Center, New York, NY, USA; Medicine, Sloan-Kettering Institute and Memorial Sloan-Kettering Cancer Center, New York, NY, USA * Corresponding author; email: d-scheinberg{at}ski.mskcc.org. Immunosuppressive agents in current use are non-specific. The capacity to delete specific CD8 T cell clones of unique specificity could prove to be a powerful tool for dissecting the precise role of CD8+ T cells in human disease and could form the basis for a safe, highly selective therapy of autoimmune disorders. MHC tetramers (multimeric complexes capable of binding to specific CD8 T cell clones) were conjugated to 225Ac (an alpha emitting atomic nanogenerator, capable of single hit killing from the cell surface) to create an agent for CD8 T cell clonal deletion. The "suicide" tetramers specifically bound to, killed, and reduced the function of their cognate CD8 T cells (either human anti-EBV or mouse anti-Listeria in two model systems) while leaving unharmed, non-specific control CD8 T cell populations. Such an approach may allow a pathway to selective ablation of pathogenic T cell clones ex vivo or in vivo without disturbing general immune function. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C. Schutz, M. Fleck, A. Mackensen, A. Zoso, D. Halbritter, J. P. Schneck, and M. Oelke Killer artificial antigen-presenting cells: a novel strategy to delete specific T cells Blood, April 1, 2008; 111(7): 3546 - 3552. [Abstract] [Full Text] [PDF] P. R. Hess, C. Barnes, M. D. Woolard, M. D. L. Johnson, J. M. Cullen, E. J. Collins, and J. A. Frelinger Selective deletion of antigen-specific CD8+ T cells by MHC class I tetramers coupled to the type I ribosome-inactivating protein saporin Blood, April 15, 2007; 109(8): 3300 - 3307. [Abstract] [Full Text] [PDF]

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