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Blood, 15 November 2004, Vol. 104, No. 10, pp. 3249-3256. Prepublished online as a Blood First Edition Paper on July 22, 2004; DOI 10.1182/blood-2004-01-0365. This Article Full Text (PDF) All Versions of this Article: 2004-01-0365v1 104/10/3249    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Weiss, L. Articles by Levy, Y. Search for Related Content PubMed PubMed Citation Articles by Weiss, L. Articles by Levy, Y. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted January 30, 2004 Accepted May 25, 2004 Human immunodeficiency virus-driven expansion of CD4+CD25+ Regulatory T cells Which Suppress HIV-specific CD4 T-cell Responses in HIV-infected Patients Laurence Weiss*, Vladimira Donkova-Petrini, Laure Caccavelli, Michele Balbo, Cedric Carbonneil, and Yves Levy INSERM U430 and University Paris V, Paris, France; Department of Immunology, Hopital Europeen Georges Pompidou, Paris, France INSERM U430 and University Paris V, Paris, France INSERM U421, University Paris XII, Creteil, France INSERM U421, University Paris XII, Creteil, France; Service of Clinical Immunology, Hopital Henri Mondor, Creteil, France * Corresponding author; email: laurence.weiss{at}egp.ap-hop-paris.fr. The present study demonstrates that CD4+CD25+ T cells, expanded in peripheral blood of HIV-infected patients receiving highly active antiretroviral therapy (HAART), exhibit phenotypic, molecular and functional characteristics of regulatory T cells. The majority of peripheral CD4+CD25+ T cells from HIV-infected patients expressed a memory phenotype. They were found to constitutively express Foxp3 messengers. CD4+CD25+ T cells weakly proliferated to immobilized anti-CD3 mAb and addition of soluble anti-CD28 mAb significantly increased proliferation. In contrast to CD4+CD25- T cells, CD4+CD25+ T cells from HIV-infected patients did not proliferate in response to recall antigens and to p24 protein. The proliferative capacity of CD4 T cells to tuberculin, CMV and p24 significantly increased following depletion of CD4+CD25+ T cells. Furthermore, addition of increasing numbers of CD4+CD25+ T cells resulted in a dose-dependent inhibition of CD4+CD25- T cell proliferation to tuberculin and p24. CD4+CD25+ T cells responded specifically to p24 antigen stimulation by expressing TGF- and IL-10, thus indicating the presence of p24-specific CD4+ T cells among the CD4+CD25+ T-cell subset. Suppressive activity was not dependent on the secretion of TGF- or IL-10. Taken together, our results suggest that persistence of HIV antigens might trigger the expansion of CD4+CD25+ regulatory T cells which might induce a tolerance to HIV in vivo. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. E. Moreno-Fernandez, W. Zapata, J. T. Blackard, G. Franchini, and C. A. Chougnet Human Regulatory T Cells Are Targets for Human Immunodeficiency Virus (HIV) Infection, and Their Susceptibility Differs Depending on the HIV Type 1 Strain J. 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