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Blood, 1 July 2004, Vol. 104, No. 1, pp. 81-88. Prepublished online as a Blood First Edition Paper on March 11, 2004; DOI 10.1182/blood-2004-01-0373. This Article Full Text (PDF) All Versions of this Article: 2004-01-0373v1 104/1/81    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Steidl, U. Articles by Kronenwett, R. Search for Related Content PubMed PubMed Citation Articles by Steidl, U. Articles by Kronenwett, R. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted January 29, 2004 Accepted February 28, 2004 Primary Human CD34+ Hematopoietic Stem and Progenitor Cells Express Functionally Active Receptors of Neuromediators Ulrich Steidl*, Simone Bork, Sebastian Schaub, Oliver Selbach, Janette Seres, Manuel Aivado, Thomas Schroeder, Ulrich P Rohr, Roland Fenk, Slawomir Kliszewski, Christian Maercker, Peter Neubert, Stefan R Bornstein, Helmut L Haas, Guido Kobbe, Daniel G Tenen, Rainer Haas, and Ralf Kronenwett Dept. of Hematology, Oncology, and Clinical Immunology, Heinrich Heine University Duesseldorf, Duesseldorf, Germany; Harvard Institutes of Medicine, Boston, MA, USA Dept. of Hematology, Oncology, and Clinical Immunology, Heinrich Heine University Duesseldorf, Duesseldorf, Germany Dept. of Neurophysiology, Heinrich Heine University Duesseldorf, Duesseldorf, Germany Dept. of Endocrinology, Heinrich Heine University Duesseldorf, Duesseldorf, Germany Harvard Institutes of Medicine, Boston, MA, USA German Resource Center for Genome Research, Berlin/Heidelberg, Germany * Corresponding author; email: usteidl{at}bidmc.harvard.edu. Recently, overlapping molecular phenotypes of hematopoietic and neuropoietic cells were described in mice. Here, we examined primary human CD34+ hematopoietic stem and progenitor cells applying specialized cDNA arrays, real-time RT-PCR, and FACS analysis focusing on genes involved in neurobiological functions. We found expression of vesicle fusion and motility genes, ligand- and voltage-gated ion channels, receptor kinases and phosphatases, and most interestingly, mRNA as well as protein expression of G protein-coupled receptors of neuromediators (CRH 1 and 2 receptors, orexin/hypocretin 1 and 2 receptors, GABA B receptor, adenosine A2B receptor, opioid 1 and µ1 receptors, 5-HT 1F receptor). As shown by two-color immunofluorescence the protein expression of these receptors was higher in the more immature CD38dim than in the CD38bright subset within the CD34+ population, and completely absent in fully differentiated blood cells, suggesting that those receptors play a role in developmentally early CD34+ stem and progenitor cells. The intracellular concentration of cAMP in CD34+ cells was diminished significantly upon stimulation of either CRH or orexin receptors indicating that those are functionally active and coupled to inhibitory G proteins in human hematopoietic cells. In conclusion, these findings suggest a molecular interrelation of neuronal and hematopoietic signaling mechanisms in humans. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Blood cells: excitable at last Richard Sullivan Blood 2004 104: 5-6. [Full Text] [PDF] This article has been cited by other articles: S. Z. Young and A. Bordey GABA's Control of Stem and Cancer Cell Proliferation in Adult Neural and Peripheral Niches Physiology, June 1, 2009; 24(3): 171 - 185. [Abstract] [Full Text] [PDF] H. L. Haas, O. A. Sergeeva, and O. Selbach Histamine in the Nervous System Physiol Rev, July 1, 2008; 88(3): 1183 - 1241. [Abstract] [Full Text] [PDF] W. Zhang, I. Hirschler-Laszkiewicz, Q. Tong, K. Conrad, S.-C. Sun, L. Penn, D. L. Barber, R. Stahl, D. J. Carey, J. Y. Cheung, et al. TRPM2 is an ion channel that modulates hematopoietic cell death through activation of caspases and PARP cleavage Am J Physiol Cell Physiol, April 1, 2006; 290(4): C1146 - C1159. [Abstract] [Full Text] [PDF]

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