SEARCH:   Advanced

Blood, 15 November 2004, Vol. 104, No. 10, pp. 3267-3275. Prepublished online as a Blood First Edition Paper on July 8, 2004; DOI 10.1182/blood-2004-01-0380. This Article Full Text (PDF) All Versions of this Article: 2004-01-0380v1 104/10/3267    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Borg, C. Articles by Figdor, C. Search for Related Content PubMed PubMed Citation Articles by Borg, C. Articles by Figdor, C. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted January 29, 2004 Accepted June 1, 2004 NK Cell Activation by Dendritic Cells (DC) Require The Formation of a Synapse leading to IL-12 Polarization in DC Christophe Borg*, Jalil Abdelali, Diego Laderach, Kouji Maruyama, Hiro Wakasugi, Sabine Charrier, Bernhard Ryffel, William Vainchenker, Anne Galy, Anne Caignard, Laurence Zitvogel, Alessandra Cambi, and Carl Figdor ERM0208 INSERM, Department of Clinical Biology, Institut Gustave Roussy, Villejuif, Val de marne, France U487 INSERM, Institut Gustave Roussy, Villejuif, France U362 INSERM, Institut Gustave Roussy, Villejuif, France; Genethon, Evry, Val de marne, France Pharmacology Division, National Cancer Center, Tokyo, Japan GEM2358, CNRS, Orleans, France U362 INSERM, Institut Gustave Roussy, Villejuif, France University Medical Center, Nijmegen, The Netherlands * Corresponding author; email: christophe.borg{at}wanadoo.fr. Mature dendritic cells (DC) can trigger the effector functions of natural killer (NK) cells. Knock-out, small interfering RNA or neutralizing antibodies targeting IL-12 subunits revealed a critical role for IL-12 in NK cell IFN secretion promoted by mature DC. However, NK cell activation by DC also required direct cell-to-cell contacts. DC-mediated NK cell activation involved the formation of stimulatory synapses between DC and NK cells. The formation of DC/NK cell conjugates was found to depend on cytoskeleton remodeling and lipid raft mobilization in DC. Moreover, the disruption of the DC cytoskeleton using pharmacological agents or the loss-of-function mutation of the Wiskott-Aldrich syndrome protein abolished the DC-mediated NK cell activation. Synapse formation promoted the polarized secretion of preassembled stores of IL-12 by DC towards the NK cell. The synaptic delivery of IL-12 by DC was found to be required for IFN secretion by NK cells, as assessed using inhibitors of cytoskeleton rearrangements and transwell experiments. Therefore, the cross talk between DC and NK cells is dictated by functional synapses. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: The yin-yang in immunity Bo Dupont Blood 2004 104: 3002-3003. [Full Text] [PDF] This article has been cited by other articles: G. Gruenbacher, H. Gander, A. Rahm, W. Nussbaumer, N. Romani, and M. Thurnher CD56+ human blood dendritic cells effectively promote TH1-type {gamma}{delta} T-cell responses Blood, November 12, 2009; 114(20): 4422 - 4431. [Abstract] [Full Text] [PDF] S. J. Conry, K. A. Milkovich, N. L. Yonkers, B. Rodriguez, H. B. Bernstein, R. Asaad, F. P. Heinzel, M. Tary-Lehmann, M. M. Lederman, and D. D. Anthony Impaired Plasmacytoid Dendritic Cell (PDC)-NK Cell Activity in Viremic Human Immunodeficiency Virus Infection Attributable to Impairments in both PDC and NK Cell Function J. Virol., November 1, 2009; 83(21): 11175 - 11187. [Abstract] [Full Text] [PDF] H. Beuneu, J. Deguine, B. Breart, O. Mandelboim, J. P. Di Santo, and P. Bousso Dynamic behavior of NK cells during activation in lymph nodes Blood, October 8, 2009; 114(15): 3227 - 3234. [Abstract] [Full Text] [PDF] C. Menard, J.-Y. Blay, C. Borg, S. Michiels, F. Ghiringhelli, C. Robert, C. Nonn, N. Chaput, J. Taieb, N. F. Delahaye, et al. Natural Killer Cell IFN-{gamma} Levels Predict Long-term Survival with Imatinib Mesylate Therapy in Gastrointestinal Stromal Tumor-Bearing Patients Cancer Res., April 15, 2009; 69(8): 3563 - 3569. [Abstract] [Full Text] [PDF] N. Lapaque, T. Walzer, S. Meresse, E. Vivier, and J. Trowsdale Interactions between Human NK Cells and Macrophages in Response to Salmonella Infection J. Immunol., April 1, 2009; 182(7): 4339 - 4348. [Abstract] [Full Text] [PDF] A. Saez-Borderias, N. Romo, G. Magri, M. Guma, A. Angulo, and M. Lopez-Botet IL-12-Dependent Inducible Expression of the CD94/NKG2A Inhibitory Receptor Regulates CD94/NKG2C+ NK Cell Function J. Immunol., January 15, 2009; 182(2): 829 - 836. [Abstract] [Full Text] [PDF] J. R. Pallandre, K. Krzewski, R. Bedel, B. Ryffel, A. Caignard, P. S. Rohrlich, X. Pivot, P. Tiberghien, L. Zitvogel, J. L. Strominger, et al. Dendritic cell and natural killer cell cross-talk: a pivotal role of CX3CL1 in NK cytoskeleton organization and activation Blood, December 1, 2008; 112(12): 4420 - 4424. [Abstract] [Full Text] [PDF] A. Iannello, O. Debbeche, S. Samarani, and A. Ahmad Antiviral NK cell responses in HIV infection: II. viral strategies for evasion and lessons for immunotherapy and vaccination J. Leukoc. Biol., July 1, 2008; 84(1): 27 - 49. [Abstract] [Full Text] [PDF] T. Strowig, F. Brilot, and C. Munz Noncytotoxic Functions of NK Cells: Direct Pathogen Restriction and Assistance to Adaptive Immunity J. Immunol., June 15, 2008; 180(12): 7785 - 7791. [Abstract] [Full Text] [PDF] Z. Tu, A. Bozorgzadeh, R. H. Pierce, J. Kurtis, I. N. Crispe, and M. S. Orloff TLR-dependent cross talk between human Kupffer cells and NK cells J. Exp. Med., January 21, 2008; 205(1): 233 - 244. [Abstract] [Full Text] [PDF] P. Colmenero, A. L. Zhang, T. Qian, L. Lu, H. Cantor, K. Soderstrom, and E. G. Engleman Qa-1b-Dependent Modulation of Dendritic Cell and NK Cell Cross-Talk In Vivo J. Immunol., October 1, 2007; 179(7): 4608 - 4615. [Abstract] [Full Text] [PDF] F. B. Thoren, A. Betten, A. I. Romero, and K. Hellstrand Cutting Edge: Antioxidative Properties of Myeloid Dendritic Cells: Protection of T Cells and NK Cells from Oxygen Radical-Induced Inactivation and Apoptosis J. Immunol., July 1, 2007; 179(1): 21 - 25. [Abstract] [Full Text] [PDF] H. Guan, M. Moretto, D. J. Bzik, J. Gigley, and I. A. Khan NK Cells Enhance Dendritic Cell Response against Parasite Antigens via NKG2D Pathway J. Immunol., July 1, 2007; 179(1): 590 - 596. [Abstract] [Full Text] [PDF] C. Liu, S. Yu, J. Kappes, J. Wang, W. E. Grizzle, K. R. Zinn, and H.-G. Zhang Expansion of spleen myeloid suppressor cells represses NK cell cytotoxicity in tumor-bearing host Blood, May 15, 2007; 109(10): 4336 - 4342. [Abstract] [Full Text] [PDF] S. Nedvetzki, S. Sowinski, R. A. Eagle, J. Harris, F. Vely, D. Pende, J. Trowsdale, E. Vivier, S. Gordon, and D. M. Davis Reciprocal regulation of human natural killer cells and macrophages associated with distinct immune synapses Blood, May 1, 2007; 109(9): 3776 - 3785. [Abstract] [Full Text] [PDF] J. Xu, A. K. Chakrabarti, J. L. Tan, L. Ge, A. Gambotto, and N. L. Vujanovic Essential role of the TNF-TNFR2 cognate interaction in mouse dendritic cell-natural killer cell crosstalk Blood, April 15, 2007; 109(8): 3333 - 3341. [Abstract] [Full Text] [PDF] S. Trifari, G. Sitia, A. Aiuti, S. Scaramuzza, F. Marangoni, L. G. Guidotti, S. Martino, P. Saracco, L. D. Notarangelo, M.-G. Roncarolo, et al. Defective Th1 Cytokine Gene Transcription in CD4+ and CD8+ T Cells from Wiskott-Aldrich Syndrome Patients J. Immunol., November 15, 2006; 177(10): 7451 - 7461. [Abstract] [Full Text] [PDF] D. Mavilio, G. Lombardo, A. Kinter, M. Fogli, A. La Sala, S. Ortolano, A. Farschi, D. Follmann, R. Gregg, C. Kovacs, et al. Characterization of the defective interaction between a subset of natural killer cells and dendritic cells in HIV-1 infection J. Exp. Med., October 2, 2006; 203(10): 2339 - 2350. [Abstract] [Full Text] [PDF] F. Miro, C. Nobile, N. Blanchard, M. Lind, O. Filipe-Santos, C. Fieschi, A. Chapgier, G. Vogt, L. de Beaucoudrey, D. S. Kumararatne, et al. T Cell-Dependent Activation of Dendritic Cells Requires IL-12 and IFN-{gamma} Signaling in T Cells J. Immunol., September 15, 2006; 177(6): 3625 - 3634. [Abstract] [Full Text] [PDF] H. Sjolin, S. H. Robbins, G. Bessou, A. Hidmark, E. Tomasello, M. Johansson, H. Hall, F. Charifi, G. B. K. Hedestam, C. A. Biron, et al. DAP12 Signaling Regulates Plasmacytoid Dendritic Cell Homeostasis and Down-Modulates Their Function during Viral Infection. J. Immunol., September 1, 2006; 177(5): 2908 - 2916. [Abstract] [Full Text] [PDF] B. Treanor, P. M.P. Lanigan, S. Kumar, C. Dunsby, I. Munro, E. Auksorius, F. J. Culley, M. A. Purbhoo, D. Phillips, M. A.A. Neil, et al. Microclusters of inhibitory killer immunoglobulin-like receptor signaling at natural killer cell immunological synapses J. Cell Biol., July 3, 2006; 174(1): 153 - 161. [Abstract] [Full Text] [PDF] P. J. O'Connell, X. Wang, M. Leon-Ponte, C. Griffiths, S. C. Pingle, and G. P. Ahern A novel form of immune signaling revealed by transmission of the inflammatory mediator serotonin between dendritic cells and T cells Blood, February 1, 2006; 107(3): 1010 - 1017. [Abstract] [Full Text] [PDF] C. Cebo, S. Da Rocha, S. Wittnebel, A. G. Turhan, J. Abdelali, S. Caillat-Zucman, J. H. Bourhis, S. Chouaib, and A. Caignard The Decreased Susceptibility of Bcr/Abl Targets to NK Cell-Mediated Lysis in Response to Imatinib Mesylate Involves Modulation of NKG2D Ligands, GM1 Expression, and Synapse Formation J. Immunol., January 15, 2006; 176(2): 864 - 872. [Abstract] [Full Text] [PDF] M. I. Lutskiy, D. S. Beardsley, F. S. Rosen, and E. Remold-O'Donnell Mosaicism of NK cells in a patient with Wiskott-Aldrich syndrome Blood, October 15, 2005; 106(8): 2815 - 2817. [Abstract] [Full Text] [PDF] T. Walzer, M. Dalod, S. H. Robbins, L. Zitvogel, and E. Vivier Natural-killer cells and dendritic cells: "l'union fait la force" Blood, October 1, 2005; 106(7): 2252 - 2258. [Abstract] [Full Text] [PDF] M. Vitale, M. D. Chiesa, S. Carlomagno, D. Pende, M. Arico, L. Moretta, and A. Moretta NK-dependent DC maturation is mediated by TNF{alpha} and IFN{gamma} released upon engagement of the NKp30 triggering receptor Blood, July 15, 2005; 106(2): 566 - 571. [Abstract] [Full Text] [PDF] C. Semino, G. Angelini, A. Poggi, and A. Rubartelli NK/iDC interaction results in IL-18 secretion by DCs at the synaptic cleft followed by NK cell activation and release of the DC maturation factor HMGB1 Blood, July 15, 2005; 106(2): 609 - 616. [Abstract] [Full Text] [PDF] M. Schmitz, S. Zhao, Y. Deuse, K. Schakel, R. Wehner, H. Wohner, K. Holig, F. Wienforth, A. Kiessling, M. Bornhauser, et al. Tumoricidal Potential of Native Blood Dendritic Cells: Direct Tumor Cell Killing and Activation of NK Cell-Mediated Cytotoxicity J. Immunol., April 1, 2005; 174(7): 4127 - 4134. [Abstract] [Full Text] [PDF] G. Mazzolini, C. Alfaro, B. Sangro, E. Feijoo, J. Ruiz, A. Benito, I. Tirapu, A. Arina, J. Sola, M. Herraiz, et al. Intratumoral Injection of Dendritic Cells Engineered to Secrete Interleukin-12 by Recombinant Adenovirus in Patients With Metastatic Gastrointestinal Carcinomas J. Clin. Oncol., February 10, 2005; 23(5): 999 - 1010. [Abstract] [Full Text] [PDF]

	Copyright © 2004 by American Society of Hematology         Online ISSN: 1528-0020