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Blood, 15 December 2004, Vol. 104, No. 13, pp. 4054-4062. Prepublished online as a Blood First Edition Paper on August 17, 2004; DOI 10.1182/blood-2004-01-0386. This Article Full Text (PDF) All Versions of this Article: 2004-01-0386v1 104/13/4054    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Koefoed, K. Articles by Ditzel, H. J Search for Related Content PubMed PubMed Citation Articles by Koefoed, K. Articles by Ditzel, H. J Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted February 4, 2004 Accepted July 28, 2004 Identification of talin head domain as an immunodominant epitope of the anti-platelet antibody response in patients with HIV-1-associated thrombocytopenia Klaus Koefoed and Henrik J Ditzel* Department of Immunology, The Scripps Research Institute, La Jolla, CA, USA Department of Immunology, The Scripps Research Institute, La Jolla, CA, USA; The Medical Biotechnology Center, Institute of Medical Biology, University of Southern Denmark, Odense, Denmark; Department of Internal Medicine C, Odense University Hospital, Odense, Denmark * Corresponding author; email: hditzel{at}health.sdu.dk. HIV-1-associated thrombocytopenia (HIV-1-ITP) is a common complication of HIV-1 infection, frequently caused by increased peripheral platelet destruction mediated by anti-platelet antibodies (Ab) and/or platelet-bound immune complexes. Little is known about the specificity of the anti-platelet Abs at a molecular level. Here, we used IgG phage display libraries generated from three HIV-1-ITP patients to isolate a large panel of human monoclonal anti-platelet Abs by selection on unfixed platelets. The platelet antigen recognized by all the cloned Abs was identified to be the talin head domain (talin-H), a cleavage product of talin, which can be generated by platelet activation or HIV-1 protease. Talin-H was found in HIV-1-ITP circulating immune complexes, and anti-talin Abs were detected in HIV-ITP sera, but not in controls. The cloned anti-talin-H IgGs were highly somatically-mutated, indicative of an antigen-driven, affinity-matured response. These findings suggest that talin-H Ab may be a marker of HIV-ITP elicited due to exposure of immunodominant epitopes on talin-H as a result of a disease-related process. Abs to talin-H and related ICs may contribute to HIV-1-ITP. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C. G. Jakobsen, N. Rasmussen, A.-V. Laenkholm, and H. J. Ditzel Phage Display Derived Human Monoclonal Antibodies Isolated by Binding to the Surface of Live Primary Breast Cancer Cells Recognize GRP78 Cancer Res., October 1, 2007; 67(19): 9507 - 9517. [Abstract] [Full Text] [PDF]

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