Blood online
Home About Blood Authors Subscriptions Permission Advertising Public Access contact us
 

 
Advanced
Current Issue
First Edition
Archives
Submit to Blood
Search
American Society of Hematology
Meeting Abstracts
Email Alerts
 Blood, 1 December 2004, Vol. 104, No. 12, pp. 3655-3663.
Prepublished online as a Blood First Edition Paper on August 17, 2004; DOI 10.1182/blood-2004-02-0412.

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
2004-02-0412v1
104/12/3655    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Right arrow Rights and Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Platzer, B.
Right arrow Articles by Strobl, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Platzer, B.
Right arrow Articles by Strobl, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

arrow to previous article Previous Article  |  Next Article next article arrow

Submitted February 2, 2004
Accepted July 15, 2004

RelB Regulates Human Dendritic Cell Subset Development by Promoting Monocyte Intermediates

Barbara Platzer, Almut Jorgl, Sabine Taschner, Bernhard Hocher, and Herbert Strobl*

Institute of Immunology, University of Vienna, Vienna, Austria

* Corresponding author; email: herbert.strobl{at}univie.ac.at.

In humans, epithelial Langerhans cells (LCs) and monocyte-derived/interstitial dendritic cells (DCs) constitute two myeloid DC sublineages. Molecular mechanisms involved in their development from common myeloid progenitors remain poorly defined. Here we demonstrate that the NF-{kappa}B transcription factor RelB regulates the generation of monocytic CD14+CD11b+ precursors of interstitial DC from human hematopoietic progenitors. RelB overexpression promoted, whereas inhibition of endogenous RelB (by p100DN) blocked precursor cell development along this DC subset pathway. Inhibition of RelB specifically arrested precursor progression from CD14loCD11b- to CD14+CD11b+ stages. Precursors were still capable of LC and granulocyte differentiation, but defective in macrophage colony-stimulating factor (M-CSF)-dependent monocyte/macrophage differentiation. RelB inhibition furthermore markedly differed from inhibition of classical NF-{kappa}B signaling, since I{kappa}B{alpha}-superrepressor (I{kappa}B{alpha}-SR) but not p100DN impaired both LC/DC differentiation, DC adhesion and progenitor cell proliferation. Furthermore, despite RelB upregulation and nuclear translocation is regarded a hallmark of human myeloid DC maturation, RelB overexpression failed to promote DC maturation. Our results suggest that RelB regulates human monopoiesis and monocyte-derived DC subset development.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Vaira, T. Johnson, A. C. Hirbe, M. Alhawagri, I. Anwisye, B. Sammut, J. O'Neal, W. Zou, K. N. Weilbaecher, R. Faccio, et al.
RelB is the NF-{kappa}B subunit downstream of NIK responsible for osteoclast differentiation
PNAS, March 11, 2008; 105(10): 3897 - 3902.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
Z. U. A. Mollah, S. Pai, C. Moore, B. J. O'Sullivan, M. J. Harrison, J. Peng, K. Phillips, J. B. Prins, J. Cardinal, and R. Thomas
Abnormal NF-{kappa}B Function Characterizes Human Type 1 Diabetes Dendritic Cells and Monocytes
J. Immunol., March 1, 2008; 180(5): 3166 - 3175.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. Taschner, C. Koesters, B. Platzer, A. Jorgl, W. Ellmeier, T. Benesch, and H. Strobl
Down-regulation of RXR{alpha} expression is essential for neutrophil development from granulocyte/monocyte progenitors
Blood, February 1, 2007; 109(3): 971 - 979.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
A. Jorgl, B. Platzer, S. Taschner, L. X. Heinz, B. Hocher, P. M. Reisner, F. Gobel, and H. Strobl
Human Langerhans-cell activation triggered in vitro by conditionally expressed MKK6 is counterregulated by the downstream effector RelB
Blood, January 1, 2007; 109(1): 185 - 193.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
S. J. A. M. Santegoets, A. J. Masterson, P. C. van der Sluis, S. M. Lougheed, D. M. Fluitsma, A. J. M. van den Eertwegh, H. M. Pinedo, R. J. Scheper, and T. D. de Gruijl
A CD34+ human cell line model of myeloid dendritic cell differentiation: evidence for a CD14+CD11b+ Langerhans cell precursor
J. Leukoc. Biol., December 1, 2006; 80(6): 1337 - 1344.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
L. X. Heinz, B. Platzer, P. M. Reisner, A. Jorgl, S. Taschner, F. Gobel, and H. Strobl
Differential involvement of PU.1 and Id2 downstream of TGF-beta1 during Langerhans-cell commitment
Blood, February 15, 2006; 107(4): 1445 - 1453.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
P. J. Cejas, L. M. Carlson, D. Kolonias, J. Zhang, I. Lindner, D. D. Billadeau, L. H. Boise, and K. P. Lee
Regulation of RelB Expression during the Initiation of Dendritic Cell Differentiation
Mol. Cell. Biol., September 1, 2005; 25(17): 7900 - 7916.
[Abstract] [Full Text] [PDF]


click for free articles
home about blood authors subscriptions permissions advertising public access contact us
  Copyright © 2004 by American Society of Hematology         Online ISSN: 1528-0020