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Blood, 1 December 2004, Vol. 104, No. 12, pp. 3655-3663. Prepublished online as a Blood First Edition Paper on August 17, 2004; DOI 10.1182/blood-2004-02-0412. This Article Full Text (PDF) All Versions of this Article: 2004-02-0412v1 104/12/3655    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Platzer, B. Articles by Strobl, H. Search for Related Content PubMed PubMed Citation Articles by Platzer, B. Articles by Strobl, H. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted February 2, 2004 Accepted July 15, 2004 RelB Regulates Human Dendritic Cell Subset Development by Promoting Monocyte Intermediates Barbara Platzer, Almut Jorgl, Sabine Taschner, Bernhard Hocher, and Herbert Strobl* Institute of Immunology, University of Vienna, Vienna, Austria * Corresponding author; email: herbert.strobl{at}univie.ac.at. In humans, epithelial Langerhans cells (LCs) and monocyte-derived/interstitial dendritic cells (DCs) constitute two myeloid DC sublineages. Molecular mechanisms involved in their development from common myeloid progenitors remain poorly defined. Here we demonstrate that the NF-B transcription factor RelB regulates the generation of monocytic CD14+CD11b+ precursors of interstitial DC from human hematopoietic progenitors. RelB overexpression promoted, whereas inhibition of endogenous RelB (by p100DN) blocked precursor cell development along this DC subset pathway. Inhibition of RelB specifically arrested precursor progression from CD14loCD11b- to CD14+CD11b+ stages. Precursors were still capable of LC and granulocyte differentiation, but defective in macrophage colony-stimulating factor (M-CSF)-dependent monocyte/macrophage differentiation. RelB inhibition furthermore markedly differed from inhibition of classical NF-B signaling, since IB-superrepressor (IB-SR) but not p100DN impaired both LC/DC differentiation, DC adhesion and progenitor cell proliferation. Furthermore, despite RelB upregulation and nuclear translocation is regarded a hallmark of human myeloid DC maturation, RelB overexpression failed to promote DC maturation. Our results suggest that RelB regulates human monopoiesis and monocyte-derived DC subset development. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: F. Gobel, S. Taschner, J. Jurkin, S. Konradi, C. Vaculik, S. Richter, D. Kneidinger, C. Muhlbacher, C. Bieglmayer, A. Elbe-Burger, et al. Reciprocal role of GATA-1 and vitamin D receptor in human myeloid dendritic cell differentiation Blood, October 29, 2009; 114(18): 3813 - 3821. [Abstract] [Full Text] [PDF] B. Platzer, S. Richter, D. Kneidinger, D. Waltenberger, M. Woisetschlager, and H. Strobl Aryl Hydrocarbon Receptor Activation Inhibits In Vitro Differentiation of Human Monocytes and Langerhans Dendritic Cells J. Immunol., July 1, 2009; 183(1): 66 - 74. [Abstract] [Full Text] [PDF] S. J. A. M. Santegoets, A. J. M. van den Eertwegh, A. A. van de Loosdrecht, R. J. Scheper, and T. D. de Gruijl Human dendritic cell line models for DC differentiation and clinical DC vaccination studies J. Leukoc. Biol., December 1, 2008; 84(6): 1364 - 1373. 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Strobl Human Langerhans-cell activation triggered in vitro by conditionally expressed MKK6 is counterregulated by the downstream effector RelB Blood, January 1, 2007; 109(1): 185 - 193. [Abstract] [Full Text] [PDF] S. J. A. M. Santegoets, A. J. Masterson, P. C. van der Sluis, S. M. Lougheed, D. M. Fluitsma, A. J. M. van den Eertwegh, H. M. Pinedo, R. J. Scheper, and T. D. de Gruijl A CD34+ human cell line model of myeloid dendritic cell differentiation: evidence for a CD14+CD11b+ Langerhans cell precursor J. Leukoc. Biol., December 1, 2006; 80(6): 1337 - 1344. [Abstract] [Full Text] [PDF] L. X. Heinz, B. Platzer, P. M. Reisner, A. Jorgl, S. Taschner, F. Gobel, and H. Strobl Differential involvement of PU.1 and Id2 downstream of TGF-beta1 during Langerhans-cell commitment Blood, February 15, 2006; 107(4): 1445 - 1453. [Abstract] [Full Text] [PDF] P. J. Cejas, L. M. Carlson, D. Kolonias, J. Zhang, I. Lindner, D. D. Billadeau, L. H. Boise, and K. P. 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