SEARCH:   Advanced

Blood, 15 August 2004, Vol. 104, No. 4, pp. 1017-1024. Prepublished online as a Blood First Edition Paper on May 6, 2004; DOI 10.1182/blood-2004-02-0419. This Article Full Text (PDF) All Versions of this Article: 2004-02-0419v1 104/4/1017    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Geddis, A. E Articles by Kaushansky, K. Search for Related Content PubMed PubMed Citation Articles by Geddis, A. E Articles by Kaushansky, K. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted February 3, 2004 Accepted April 20, 2004 Megakaryocytes express functional aurora-B kinase in endomitosis Amy E Geddis* and Ken Kaushansky Medicine, University of California San Diego, San Diego, CA, USA Pediatrics, University of California San Diego, San Diego, CA, USA * Corresponding author; email: ageddis{at}ucsd.edu. Endomitosis (EnM) in megakaryocytes (MKs) is characterized by abortion of mitosis in late anaphase and failure of cytokinesis; subsequent reinitiation of DNA synthesis results in polyploidy. Ablation of chromosomal passenger proteins including aurora-B kinase cause defects in late anaphase and cytokinesis in diploid cells; thus one hypothesis is that the expression or function of these proteins in polyploid MKs is abnormal. It has been reported that aurora kinase B mRNA is decreased in polyploid megakaryocytic cells, suggesting that deficiency of aurora-B kinase is responsible for EnM. We examined the localization of aurora-B kinase and additional members of the chromosomal passenger protein and aurora kinase families in MKs. We found that in EnM MKs 1) Aurora-B kinase is present and appropriately localized to centromeres in early EnM; 2) in low ploidy human MKs, centromeric localization of survivin and INCENP can also be demonstrated; 3) the function of aurora-B kinase, as measured by serine10 phosphorylation of histone H3, is intact; and 4) aurora-A kinase localizes appropriately to centrosomes in EnM. These results suggest that EnM MKs appropriately express functional aurora-B kinase and related proteins in early anaphase, making a simple deficiency of this protein an unlikely explanation for polyploidy in this cell type. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: Q. Wen, C. Leung, Z. Huang, S. Small, A. L. Reddi, J. D. Licht, and J. D. Crispino Survivin is not required for the endomitotic cell cycle of megakaryocytes Blood, July 2, 2009; 114(1): 153 - 156. [Abstract] [Full Text] [PDF] L. Lordier, A. Jalil, F. Aurade, F. Larbret, J. Larghero, N. Debili, W. Vainchenker, and Y. Chang Megakaryocyte endomitosis is a failure of late cytokinesis related to defects in the contractile ring and Rho/Rock signaling Blood, October 15, 2008; 112(8): 3164 - 3174. [Abstract] [Full Text] [PDF] E. Walsby, V. Walsh, C. Pepper, A. Burnett, and K. Mills Effects of the aurora kinase inhibitors AZD1152-HQPA and ZM447439 on growth arrest and polyploidy in acute myeloid leukemia cell lines and primary blasts Haematologica, May 1, 2008; 93(5): 662 - 669. [Abstract] [Full Text] [PDF] D. J. McCrann, T. Yezefski, H. G. Nguyen, N. Papadantonakis, H. Liu, Q. Wen, J. D. Crispino, and K. Ravid Survivin overexpression alone does not alter megakaryocyte ploidy nor interfere with erythroid/megakaryocytic lineage development in transgenic mice Blood, April 15, 2008; 111(8): 4092 - 4095. [Abstract] [Full Text] [PDF] I. S. Hitchcock, N. E. Fox, N. Prevost, K. Sear, S. J. Shattil, and K. Kaushansky Roles of focal adhesion kinase (FAK) in megakaryopoiesis and platelet function: studies using a megakaryocyte lineage specific FAK knockout Blood, January 15, 2008; 111(2): 596 - 604. [Abstract] [Full Text] [PDF] S. Fukuda and L. M. Pelus Survivin, a cancer target with an emerging role in normal adult tissues Mol. Cancer Ther., May 1, 2006; 5(5): 1087 - 1098. [Abstract] [Full Text] [PDF] A. G. Muntean and J. D. Crispino Differential requirements for the activation domain and FOG-interaction surface of GATA-1 in megakaryocyte gene expression and development Blood, August 15, 2005; 106(4): 1223 - 1231. [Abstract] [Full Text] [PDF] S. Gurbuxani, Y. Xu, G. Keerthivasan, A. Wickrema, and J. D. Crispino Differential requirements for survivin in hematopoietic cell development PNAS, August 9, 2005; 102(32): 11480 - 11485. [Abstract] [Full Text] [PDF] B. J. Lannutti, N. Blake, M. J. Gandhi, J. A. Reems, and J. G. Drachman Induction of polyploidization in leukemic cell lines and primary bone marrow by Src kinase inhibitor SU6656 Blood, May 15, 2005; 105(10): 3875 - 3878. [Abstract] [Full Text] [PDF]

	Copyright © 2004 by American Society of Hematology         Online ISSN: 1528-0020