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Blood, 15 September 2004, Vol. 104, No. 6, pp. 1648-1655. Prepublished online as a Blood First Edition Paper on June 3, 2004; DOI 10.1182/blood-2004-02-0448. This Article Full Text (PDF) All Versions of this Article: 2004-02-0448v1 104/6/1648    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Hess, D. A Articles by Nolta, J. A Search for Related Content PubMed PubMed Citation Articles by Hess, D. A Articles by Nolta, J. A Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted February 9, 2004 Accepted April 15, 2004 Functional characterization of highly purified human hematopoietic repopulating cells isolated based on aldehyde dehydrogenase activity David A Hess*, Todd E Meyerrose, Louisa Wirthlin, Timothy P Craft, Phillip E Herrbrich, Michael H Creer, and Jan A Nolta Internal Medicine, Division of Oncology, Hematopoietic Development and Malignancy Program, Washington University School of Medicine, St. Louis, MO, USA Pathology and Laboratory Medicine, Saint Louis University, St. Louis, MO, USA * Corresponding author; email: dhess{at}im.wustl.edu. Human hematopoietic stem cells (HSC) are commonly purified by the expression of cell surface markers such as CD34. Since cell phenotype can be altered by cell cycle progression or ex vivo culture, purification based on conserved stem cell function may represent a more reliable way to isolate various stem cell populations. We have purified primitive HSC from human umbilical cord blood (UCB) by lineage depletion (Lin-) followed by selection of cells with high aldehyde dehydrogenase (ALDH) activity. ALDHhiLin- cells comprised 22.6±3.0% of the Lin- population, and highly co-expressed primitive HSC phenotypes (CD34+CD38- and CD34+CD133+). In vitro hematopoietic progenitor function was enriched in the ALDHhiLin- population, compared to ALDHloLin- cells. Multilineage human hematopoietic repopulation was observed exclusively after transplantation of ALDHhiLin- cells. Direct comparison of repopulation using the NOD/SCID and NOD/SCID 2 microglobulin (2M) null models demonstrated that 10-fold greater numbers of ALDHhiLin- cells were needed to engraft the NOD/SCID mouse as compared to the more permissive NOD/SCID 2M null mouse, suggesting that the ALDHhiLin- population contained committed progenitors as well as primitive repopulating cells. Cell fractionation based on lineage depletion and ALDH activity provides a viable and prospective purification of HSC based on cell function rather than cell surface phenotype. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Vitamin A metabolism and stemness Edward F. Srour Blood 2004 104: 1597-1598. [Full Text] [PDF] This article has been cited by other articles: B. J. Capoccia, D. L. Robson, K. D. Levac, D. J. Maxwell, S. A. Hohm, M. J. Neelamkavil, G. I. Bell, A. Xenocostas, D. C. Link, D. Piwnica-Worms, et al. 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