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Blood, 15 February 2005, Vol. 105, No. 4, pp. 1807-1809. Prepublished online as a Blood First Edition Paper on October 12, 2004; DOI 10.1182/blood-2004-02-0454. This Article Full Text (PDF) All Versions of this Article: 2004-02-0454v1 105/4/1807    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Weinberg, R. S Articles by Atweh, G. F Search for Related Content PubMed PubMed Citation Articles by Weinberg, R. S Articles by Atweh, G. F Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted February 5, 2004 Accepted October 5, 2004 Butyrate increases the efficiency of translation of -Globin mRNA Rona S Weinberg*, Xijun Ji, Millicent Sutton, Susan Perrine, Yelena Galperin, Qiliang Li, Stephen A Liebhaber, George Stamatoyannopoulos, and George F Atweh Department of Medicine, Division of Hematology/Oncology, Mount Sinai School of Medicine, New York, NY, USA Departments of Genetics and Medicine, University of Pennsylvania, Philadelphia, PA, USA Hemoglobinopathy Thalassemia Research Unit, Boston University School of Medicine, Boston, MA, USA Division of Medical Genetics, University of Washington, Seattle, WA, USA * Corresponding author; email: rweinberg{at}nybloodcenter.org. Fetal hemoglobin (Hb F) levels increase in the majority of sickle cell patients following intermittent butyrate therapy. Although the full effects of butyrate on Hb F levels usually require multiple treatment cycles, in some patients, a peak level is achieved after a few days of butyrate therapy. Our investigation of the mechanism(s) responsible for this rapid induction of Hb F by butyrate showed that reticulocyte -globin chain synthesis markedly increased within 24 hours of butyrate exposure, without concomitant changes in reticulocyte -globin mRNA levels. This suggests that butyrate might induce Hb F by increasing the efficiency of translation of -globin mRNA. This hypothesis was confirmed by ribosome loading studies that demonstrated enrichment of the polysomal fraction of reticulocytes with -globin mRNA following butyrate exposure. Thus, the induction of Hb F by butyrate may be mediated by translational effects in addition to its well-known effects on transcription of the -globin genes. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: R. Mabaera, M. R. Greene, C. A. Richardson, S. J. Conine, C. D. Kozul, and C. H. Lowrey Neither DNA hypomethylation nor changes in the kinetics of erythroid differentiation explain 5-azacytidine's ability to induce human fetal hemoglobin Blood, January 1, 2008; 111(1): 411 - 420. [Abstract] [Full Text] [PDF] H. Fathallah, R. S. Weinberg, Y. Galperin, M. Sutton, and G. F. Atweh Role of epigenetic modifications in normal globin gene regulation and butyrate-mediated induction of fetal hemoglobin Blood, November 1, 2007; 110(9): 3391 - 3397. [Abstract] [Full Text] [PDF] M. J. Telen Role of Adhesion Molecules and Vascular Endothelium in the Pathogenesis of Sickle Cell Disease Hematology, January 1, 2007; 2007(1): 84 - 90. [Abstract] [Full Text] [PDF] J. Sangerman, M. S. Lee, X. Yao, E. Oteng, C.-H. Hsiao, W. Li, S. Zein, S. F. Ofori-Acquah, and B. S. Pace Mechanism for fetal hemoglobin induction by histone deacetylase inhibitors involves {gamma}-globin activation by CREB1 and ATF-2 Blood, November 15, 2006; 108(10): 3590 - 3599. [Abstract] [Full Text] [PDF] R. Mankidy, D. V. Faller, R. Mabaera, C. H. Lowrey, M. S. Boosalis, G. L. White, S. A. Castaneda, and S. P. Perrine Short-chain fatty acids induce {gamma}-globin gene expression by displacement of a HDAC3-NCoR repressor complex Blood, November 1, 2006; 108(9): 3179 - 3186. [Abstract] [Full Text] [PDF] A. Bank Regulation of human fetal hemoglobin: new players, new complexities Blood, January 15, 2006; 107(2): 435 - 443. [Abstract] [Full Text] [PDF] H. Fathallah and G. F. Atweh Induction of Fetal Hemoglobin in the Treatment of Sickle Cell Disease Hematology, January 1, 2006; 2006(1): 58 - 62. [Abstract] [Full Text] [PDF] H. FATHALLAH, M. SUTTON, and G. F. ATWEH Pharmacological Induction of Fetal Hemoglobin: Why Haven't We Been More Successful in Thalassemia? Ann. N.Y. Acad. Sci., November 1, 2005; 1054(1): 228 - 237. [Abstract] [Full Text] [PDF] S. P. PERRINE, S. A. CASTANEDA, M. S. BOOSALIS, G. L. WHITE, B. M. JONES, and R. BOHACEK Induction of Fetal Globin in {beta}-Thalassemia: Cellular Obstacles and Molecular Progress Ann. N.Y. Acad. Sci., November 1, 2005; 1054(1): 257 - 265. [Abstract] [Full Text] [PDF]

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