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Blood, 15 December 2004, Vol. 104, No. 13, pp. 4002-4009. Prepublished online as a Blood First Edition Paper on May 25, 2004; DOI 10.1182/blood-2004-02-0494. This Article Full Text (PDF) Supplemental Figure All Versions of this Article: 2004-02-0494v1 104/13/4002    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Gricks, C. S Articles by Gribben, J. G Search for Related Content PubMed PubMed Citation Articles by Gricks, C. S Articles by Gribben, J. G Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted February 9, 2004 Accepted May 3, 2004 Differential regulation of gene expression following CD40 activation of leukemic compared to healthy B cells Clair S Gricks, David Zahrieh, A J Zauls, Gullu Gorgun, Daniela Drandi, Katja Mauerer, Donna Neuberg, and John G Gribben* Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, USA Biostatistical Science, Dana-Farber Cancer Institute, Boston, MA, USA * Corresponding author; email: john_gribben{at}dfci.harvard.edu. It is possible to differentiate malignant from healthy cells and to classify diseases based upon identification of specific gene expression profiles. We hypothesized that gene expression profiling could also be used to identify differential activation of healthy and malignant cells, and as a model for this examined the molecular sequelae of CD40 activation of healthy and B cell chronic lymphocytic leukemia (CLL). Hierarchical clustering analysis of gene expression signatures grouped samples by CD40 activation status and further sub-classified CD40 activated CLL cells from healthy B cells. Supervised analyses in healthy B cells compared to CLL cells identified differential regulation of genes governing cell cycle progression and apoptosis. CD40 signaling of CLL cells increases their susceptibility to immune recognition, but promotes survival and cell cycle arrest, making these cells potentially more resistant to chemotherapy. These results illustrate the utility of gene expression profiling to elucidate the molecular sequelae of signaling in healthy cells and altered signaling pathways in malignant cells. This type of approach should be useful to identify targets of therapy of malignant diseases. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: All B cells are not the same: a story of CD40 activation John C. Byrd Blood 2004 104: 3847. [Full Text] [PDF] This article has been cited by other articles: M. Herling, K. A. Patel, N. Weit, N. Lilienthal, M. Hallek, M. J. Keating, and D. Jones High TCL1 levels are a marker of B-cell receptor pathway responsiveness and adverse outcome in chronic lymphocytic leukemia Blood, November 19, 2009; 114(21): 4675 - 4686. [Abstract] [Full Text] [PDF] A. G. Ramsay, A. J. Clear, G. Kelly, R. Fatah, J. Matthews, F. MacDougall, T. A. Lister, A. M. Lee, M. Calaminici, and J. G. 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