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Blood, 15 October 2004, Vol. 104, No. 8, pp. 2332-2338.
Prepublished online as a Blood First Edition Paper on July 1, 2004; DOI 10.1182/blood-2004-02-0511.
 Previous Article | Next Article 
Submitted February 10, 2004
Accepted June 10, 2004
Segregation of lipid raft markers including CD133 in polarized human hematopoietic stem and progenitor cells
Bernd Giebel*, Denis Corbeil, Julia Beckmann, Johannes Hoehn, Daniel Freund, Kay Giesen, Johannes Fischer, Gesine Koegler, and Peter Wernet
Heinrich-Heine-University Duesseldorf, Institute for Transplantations Diagnostics and Cell Therapeutics, Duesseldorf, Germany
University Carl Gustav Carus, Medical Clinic and Polyclinic I, Dresden, Germany; Max-Planck-Institute of Molecular Cell Biology and Genetics, Dresden, Germany
University Carl Gustav Carus, Medical Clinic and Polyclinic I, Dresden, Germany
* Corresponding author; email: giebel{at}itz.uni-duesseldorf.de.
During ontogenesis and the entire adult life hematopoietic stem and progenitor cells have the capability to migrate. In comparison to the process of peripheral leukocyte migration in inflammatory responses the molecular and cellular mechanisms governing the migration of these cells remain poorly understood. A common feature of migrating cells is that they need to become polarized before they migrate. Here we have investigated the issue of cell polarity of hematopoietic stem/progenitor cells in detail. We found that human CD34+ hematopoietic cells (i) acquire a polarized cell shape upon cultivation with the formation of a leading edge at the front pole and an uropod at the rear pole; (ii) exhibit an amoeboid movement, which is similar to the one described for migrating peripheral leukocytes, and (iii) redistribute several lipid raft markers including cholesterol-binding protein prominin-1 (CD133) in specialized plasma membrane domains. Furthermore, polarization of CD34+ cells is stimulated by early acting cytokines and require the activity of phosphoinositol-3-kinase as previously reported for peripheral leukocyte polarization. Together, our data reveal a strong correlation between polarization and migration of peripheral leukocytes and hematopoietic stem/progenitor cells and suggest that they are governed by similar mechanisms.
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