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Blood, 15 January 2005, Vol. 105, No. 2, pp. 830-837. Prepublished online as a Blood First Edition Paper on September 9, 2004; DOI 10.1182/blood-2004-02-0564. This Article Full Text (PDF) All Versions of this Article: 2004-02-0564v1 105/2/830    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Scapini, P. Articles by Cassatella, M. A Search for Related Content PubMed PubMed Citation Articles by Scapini, P. Articles by Cassatella, M. A Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted February 13, 2004 Accepted August 31, 2004 Proinflammatory mediators elicit the secretion of the intracellular B-lymphocyte stimulator (BLyS) pool that is stored in activated neutrophils: implications for inflammatory diseases Patrizia Scapini, Antonio Carletto, Bernardetta Nardelli, Federica Calzetti, Viktor Roschke, Flavia Merigo, Nicola Tamassia, Sara Pieropan, Domenico Biasi, Andrea Sbarbati, Silvano Sozzani, Lisa Bambara, and Marco A Cassatella* Department of Pathology, Division of General Pathology, University of Verona, Verona, Italy Department of Clinical and Experimental Medicine, Division of Rheumatology, University of Verona, Verona, Italy Human Genome Sciences, Inc., Rockville, MD, USA Department of Morphological and Biomedical Sciences, Division of Anatomy and Histology, University of Verona, Verona, Italy Department of Biotechnology, Division of General Pathology and Immunology, University of Brescia, Milano, Italy * Corresponding author; email: marco.cassatella{at}univr.it. We have recently shown that G-CSF- and IFN-activated human neutrophils accumulate and release remarkable amounts of soluble B-lymphocyte stimulator (BLyS) in vitro. In this study, we provide evidence that neutrophils migrating into skin window exudates (SWE), developed in both healthy volunteers and rheumatoid arthritis (RA) patients, synthesize and release BLyS in response to locally produced G-CSF. Accordingly, the concentrations of soluble BLyS in SWE were significantly more elevated than in serum. Because the levels of SWE BLyS, but not SWE G-CSF, were higher in RA patients than in healthy subjects, we examined the effect of CXCL8/IL-8, C5a and other proinflammatory mediators that dramatically accumulate in RA SWE and/or inflamed synovial fluids. We show that CXCL1/GRO, CXCL8/IL-8, C5a, immune complexes, TNF, leukotriene-B4, fMLP and LPS, which by themselves do not induce BLyS de novo synthesis, act as potent secretagogues for BLyS, which is mainly stored in Golgi-related compartments within G-CSF-treated neutrophils, as determined by immunogold electron microscopy. This action is pivotal in greatly amplifying neutrophil-dependent BLyS release in SWE of RA patients relative to healthy donors. Collectively, our data uncover a novel mechanism that might dramatically exacerbate the release of BLyS by neutrophils during pathological inflammatory responses. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: E. Mohr, K. Serre, R. A. Manz, A. F. Cunningham, M. Khan, D. L. Hardie, R. Bird, and I. C. M. MacLennan Dendritic Cells and Monocyte/Macrophages That Create the IL-6/APRIL-Rich Lymph Node Microenvironments Where Plasmablasts Mature J. 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