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Blood, 1 March 2005, Vol. 105, No. 5, pp. 2168-2174. Prepublished online as a Blood First Edition Paper on November 4, 2004; DOI 10.1182/blood-2004-02-0616. This Article Full Text (PDF) All Versions of this Article: 2004-02-0616v1 105/5/2168    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Koury, M. J Articles by Bondurant, M. C Search for Related Content PubMed PubMed Citation Articles by Koury, M. J Articles by Bondurant, M. C Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted February 18, 2004 Accepted October 28, 2004 In vitro maturation of nascent reticulocytes to erythrocytes Mark J Koury*, Stephen T Koury, Prapaporn Kopsombut, and Maurice C Bondurant Tennessee Valley Healthcare System Veterans Affairs Medical Center, Nashville, TN, USA; Division of Hematology/Oncology, Department of Medicine, Vanderbilt University University School of Medicine, Nashville, TN, USA Department of Biotechnical and Clinical Laboratory Sciences, University of Buffalo, Buffalo, NY, USA Division of Hematology/Oncology, Department of Medicine, Vanderbilt University University School of Medicine, Nashville, TN, USA * Corresponding author; email: mark.koury{at}vanderbilt.edu. Most studies of mammalian reticulocyte maturation have used blood reticulocytes. Nascent reticulocytes, as found in bone marrow, have not been available in developmentally synchronized populations. Nascent murine reticulocytes formed in vitro by enucleation of Friend virus-infected erythroblasts were purified and recultured for 110 hours. At 0 hours, all recultured cells were lobulated and contained dense, centralized reticulin. By 110 hours, about 20-25% of the cells became biconcave erythrocytes. Most ribosomes and cellular RNAs were degraded within 20 hours, and during that period, heme synthesis declined from a rate equal to that of late erythroblasts to less than 10% of that rate. Many mitochondria appeared normal until they showed outer membrane swelling, degradation, and apparent fusion with intracellular vacuoles at 40 hours of culture. During the period of mitochondrial loss, Bcl-XL, an antiapoptotic protein that accumulates during erythroblast differentiation and maintains mitochondrial membrane integrity, demonstrated progressive decreases and changes consistent with deamidation. Nevertheless, the reticulocytes did not undergo apoptosis because their apoptotic machinery was degraded. This experimental system that provides a developmentally synchronized population of nascent murine reticulocytes that mature into biconcave erythrocytes in vitro should be useful in further investigations of the cellular events involved in reticulocyte maturation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. Zhang, M. S. Randall, M. R. Loyd, F. C. Dorsey, M. Kundu, J. L. Cleveland, and P. A. Ney Mitochondrial clearance is regulated by Atg7-dependent and -independent mechanisms during reticulocyte maturation Blood, July 2, 2009; 114(1): 157 - 164. [Abstract] [Full Text] [PDF] I. S. Naarmann, C. Harnisch, N. Flach, E. Kremmer, H. Kuhn, D. H. Ostareck, and A. Ostareck-Lederer mRNA Silencing in Human Erythroid Cell Maturation: HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN K CONTROLS THE EXPRESSION OF ITS REGULATOR c-Src J. Biol. Chem., June 27, 2008; 283(26): 18461 - 18472. [Abstract] [Full Text] [PDF] K. E. McGrath, P. D. Kingsley, A. D. Koniski, R. L. Porter, T. P. Bushnell, and J. Palis Enucleation of primitive erythroid cells generates a transient population of "pyrenocytes" in the mammalian fetus Blood, February 15, 2008; 111(4): 2409 - 2417. [Abstract] [Full Text] [PDF] S.-H. Goh, M. Josleyn, Y. T. Lee, R. L. Danner, R. B. Gherman, M. C. Cam, and J. L. Miller The human reticulocyte transcriptome Physiol Genomics, July 18, 2007; 30(2): 172 - 178. [Abstract] [Full Text] [PDF] D. Su, J. M. May, M. J. Koury, and H. Asard Human Erythrocyte Membranes Contain a Cytochrome b561 That May Be Involved in Extracellular Ascorbate Recycling J. Biol. Chem., December 29, 2006; 281(52): 39852 - 39859. [Abstract] [Full Text] [PDF] M. M. Rhodes, P. Kopsombut, M. C. Bondurant, J. O. Price, and M. J. Koury Bcl-xL prevents apoptosis of late-stage erythroblasts but does not mediate the antiapoptotic effect of erythropoietin Blood, September 1, 2005; 106(5): 1857 - 1863. [Abstract] [Full Text] [PDF]

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